VIP Inhibits Porphyromonas gingivalis LPS-induced immune responses in human monocytes
| Autor: | Jill Cheetham, John J. Taylor, Philip M. Preshaw, Neil Foster |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Time Factors Lipopolysaccharide Proto-Oncogene Proteins c-jun CD14 Vasoactive intestinal peptide Lipopolysaccharide Receptors Inflammation Enzyme-Linked Immunosorbent Assay Monocytes 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immune system Medicine Humans General Dentistry Porphyromonas gingivalis Cells Cultured Microscopy Confocal biology business.industry Tumor Necrosis Factor-alpha Monocyte NF-kappa B 030206 dentistry Th1 Cells biology.organism_classification Flow Cytometry 030104 developmental biology medicine.anatomical_structure chemistry Immunology Cytokines Cytokine secretion medicine.symptom Inflammation Mediators business Immunosuppressive Agents Vasoactive Intestinal Peptide |
| Zdroj: | Journal of dental research. 84(11) |
| ISSN: | 0022-0345 |
| Popis: | Lipopolysaccharide (LPS) from the Gram-negative pathogen Porphyromonas gingivalis ( Pg) stimulates cytokine secretion in immune cells, and thereby initiates the inflammation associated with periodontitis. Modulation of pro-inflammatory cytokine activity is a plausible therapeutic target in periodontal disease. Vasoactive intestinal peptide (VIP) has a role in immunoregulation, and has been identified as a molecule with therapeutically beneficial immunosuppressive effects in inflammatory and autoimmune conditions. We aimed to investigate the effect of VIP on immune responses induced by Pg LPS in vitro. VIP (10−8M) significantly (P < 0.05) inhibits TNF-α production by human monocytic THP1 cells stimulated with Pg LPS. In parallel, we showed that VIP inhibits nuclear translocation of NFκB and c-Jun in a time-dependent manner, but does not decrease the expression of CD14 receptors. This is the first report to show the potential of VIP as an immunomodulator of Pg-stimulated inflammatory pathways in human monocytes. |
| Databáze: | OpenAIRE |
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