Biochemical characterisation of a Kunitz-type inhibitor from Tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
| Autor: | Daniel Nogoceke Sifuentes, Adriana F. Uchoa, Beatriz Blenda Pinheiro de Souza, Ana Heloneida de Araújo Morais, Bruna Leal Lima Maciel, Amanda Fernandes de Medeiros, Izael de Sousa Costa, Raphael Paschoal Serquiz, Elizeu Antunes dos Santos, Fabiana Maria Coimbra de Carvalho, Sumika Kiyota |
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| Přispěvatelé: | AMANDA FERNANDES DE MEDEIROS, UFRN, IZAEL DE SOUSA COSTA, UFRN, FABIANA MARIA COIMBRA DE CARVALHO, UFRN, SUMIKA KIYOTA, CPDSA, BIOLOGICAL INSTITUTE, BEATRIZ BLENDA PINHEIRO DE SOUZA, UNB, DANIEL NOGOCEKE SIFUENTES, Cenargen, RAPHAEL PASCHOAL SERQUIZ, UFRN, BRUNA LEAL LIMA MACIEL, UFRN, ADRIANA FERREIRA UCHÔA, UFRN, ELIZEU ANTUNES DOS SANTOS, UFRN, ANA HELONEIDA DE ARAÚJO MORAIS, UFRN. |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Leptin Male Antitryptic Tamarinds Trypsin inhibitor 030209 endocrinology & metabolism Tamarind High-performance liquid chromatography antitryptic 03 medical and health sciences Structure-Activity Relationship 0302 clinical medicine Drug Discovery medicine Tamarindus Structure–activity relationship Animals Trypsin Obesity Rats Wistar IC50 Cholecystokinin Plant Proteins Pharmacology Dose-Response Relationship Drug Chemistry CCK lcsh:RM1-950 General Medicine Rats Wistar rats Disease Models Animal 030104 developmental biology Isoelectric point lcsh:Therapeutics. Pharmacology Biochemistry Seeds Anti-Obesity Agents Peptides medicine.drug Research Paper |
| Zdroj: | Journal of Enzyme Inhibition and Medicinal Chemistry, Vol 33, Iss 1, Pp 334-348 (2018) Journal of Enzyme Inhibition and Medicinal Chemistry Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| ISSN: | 1475-6374 1475-6366 |
| Popis: | A trypsin inhibitor isolated from tamarind seed (TTI) has satietogenic effects in animals, increasing the cholecystokinin (CCK) in eutrophy and reducing leptin in obesity. We purified TTI (pTTI), characterised, and observed its effect upon CCK and leptin in obese Wistar rats. By HPLC, and after amplification of resolution, two protein fractions were observed: Fr1 and Fr2, with average mass of [M + 14H]+ = 19,594,690 Da and [M + 13H]+ = 19,578,266 Da, respectively. The protein fractions showed 54 and 53 amino acid residues with the same sequence. pTTI presented resistance to temperature and pH variations; IC50 was 2.7 × 10−10 mol.L−1 and Ki was 2.9 × 10−11 mol.L−1. The 2-DE revealed spots with isoelectric points between pH 5 and 6, and one near pH 8. pTTI action on leptin decrease was confirmed. We conclude that pTTI is a Kunitz trypsin inhibitor with possible biotechnological health-related application. |
| Databáze: | OpenAIRE |
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