Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis
| Autor: | Tayler Debrosse, Andrew Travers, Allan M. Showalter, Kirk Emch, Lu Tian, Hayley Herock, Emily Poirier, Debarati Basu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sucrose Cell signaling Glycosylation Mutant Arabidopsis lcsh:Medicine Protein Serine-Threonine Kinases Sodium Chloride Lignin Plant Roots 01 natural sciences Cell wall Plant Mucilage 03 medical and health sciences Plant Growth Regulators Cell Wall Gene Expression Regulation Plant lcsh:Science Cellulose Cell Proliferation Arabinogalactan protein 2. Zero hunger Multidisciplinary biology Arabidopsis Proteins Reverse Transcriptase Polymerase Chain Reaction Kinase Cell growth lcsh:R Gene Expression Regulation Developmental Receptor Protein-Tyrosine Kinases Galactosyltransferases biology.organism_classification 030104 developmental biology Biochemistry Mutation Seeds lcsh:Q Signal transduction Cell Adhesion Molecules Research Article Signal Transduction 010606 plant biology & botany |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 1, p e0145092 (2016) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0145092 |
| Popis: | Fundamental processes that underpin plant growth and development depend crucially on the action and assembly of the cell wall, a dynamic structure that changes in response to both developmental and environmental cues. While much is known about cell wall structure and biosynthesis, much less is known about the functions of the individual wall components, particularly with respect to their potential roles in cellular signaling. Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function. Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis. These mutants phenocopy a salt-overly sensitive mutant called sos5, which lacks a fasciclin-like AGP (SOS5/FLA4) as well as a fei1fei2 double mutant, which lacks two cell wall-associated leucine-rich repeat receptor-like kinases. Additionally, galt2gal5 as well as sos5 and fei2 showed reduced seed mucilage adherence. Quintuple galt2galt5sos5fei1fei2 mutants were produced and provided evidence that these genes act in a single, linear genetic pathway. Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|