Characterization of a novel polysaccharide from Ganoderma lucidum and its absorption mechanism in Caco-2 cells and mice model
| Autor: | Zichao Wang, Yingbin Shen, Xiaoxiao Zhao, Huiru Zhang, Xueqin Wang, Xiaobei Zhan, Jiaqi Wang, Kun Fan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Arabinose
endocrine system Reishi Rhamnose 02 engineering and technology Absorption (skin) Xylose Polysaccharide 01 natural sciences Biochemistry Mice chemistry.chemical_compound Polysaccharides Structural Biology In vivo Animals Humans Molecular Biology chemistry.chemical_classification biology 010405 organic chemistry digestive oral and skin physiology Water General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences Molecular Weight Glucose chemistry Caco-2 biology.protein Pinocytosis GLUT2 Caco-2 Cells 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 118:320-326 |
| ISSN: | 0141-8130 |
| Popis: | Fungal polysaccharides are mainly taken orally, so it is very important to study the absorption mechanism of which in vivo for understanding its biological activities. In this work, a polysaccharide fraction (GLP) was purified from G. lucidum by water extraction and alcohol precipitation. Physicochemical characterization indicated that GLP had a molecular weight of 108 kDa and consisted of glucose (35.56%), xylose (40.11%), rhamnose (16.45%) and l -arabinose (7.88%) in a molar radio of 4.51: 5.09: 2.88: 1.0. GLP was taken by Caco-2 cells through the pathway of macropinocytosis and it was a well-absorbed biological macromolecule testified by Papp value. Sugar transporters (SGLT1 and GLUT2) played different roles at different absorption stages of GLP, and the absorption of GLP during digestion in mice model was a time-dependent process. All these results could contribute to explore the absorption mechanism of polysaccharides in body. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect