Melatonin enhances the in vitro and in vivo repair of severed rat sciatic axons
| Autor: | Aleksej Zuzek, George D. Bittner, Joshua M. Britt, Elizabeth Truong, Ronda C. Stavisky |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Nerve Crush
medicine.medical_treatment Action Potentials macromolecular substances Pharmacology Methylprednisolone Polyethylene Glycols Melatonin Rats Sprague-Dawley Surface-Active Agents Organ Culture Techniques Neurotrophic factors In vivo Cyclosporin a PEG ratio Glial cell line-derived neurotrophic factor medicine Animals Axon Enzyme Inhibitors biology General Neuroscience Anatomy Sciatic Nerve Culture Media Nerve Regeneration Rats Electrophysiology medicine.anatomical_structure Neuroprotective Agents nervous system biology.protein Cyclosporine Calcium Axotomy medicine.drug |
| Zdroj: | Neuroscience letters. 376(2) |
| ISSN: | 0304-3940 |
| Popis: | This study examines the effects of several experimental compounds [melatonin (MEL), cyclosporin A (CsA), glial-derived neurotrophic factor (GDNF), and methylprednisolone (MP)] on polyethylene glycol (PEG)-induced repair in vitro and/or in vivo by plasmalemmal fusion (PEG-fusion) of sciatic axons severed by crushing. As measured by conduction of compound action potentials (CAPs) through the lesion site, a significantly (p0.025) higher percentage (75%) of crushed rat sciatic axons can be repaired in vitro by PEG-fusion following exposure to MEL compared to PEG-fusion of severed sciatic axons in control Krebs saline that contains calcium (CTL=20%). In contrast, no other experimental compound (GDNF: 45%; MP: 42%; CsA: 24%) produces a significant improvement in PEG-fusion success compared to CTL. Further, MEL produces significantly (p0.001) larger peak CAP amplitudes conducted through the lesion site following PEG-fusion compared to CTL or any other experimental compound in vitro. Additionally, MEL significantly (p0.025) increases the ability to PEG-fuse sciatic axons in vivo, compared to CTL. Finally, PEG-fusion success in vivo is significantly (p0.01) greater in calcium-free CTL (CTL-Ca) compared to CTL. |
| Databáze: | OpenAIRE |
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