Korean Red ginseng extract inhibits glioblastoma propagation by blocking the Wnt signaling pathway
| Autor: | Xiong Jin, Hyunggee Kim, Sunyoung Seo, Seon Yong Lee, Hee Young Jeon, Kiyoung Eun, Jung Yun Kim, Sang Hun Choi, Yong Yook Lee, Cheol Gyu Park, Jun Kyum Kim, Nayoung Hong, Eun Jung Kim, Seok Won Ham |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Programmed cell death Panax Biology 03 medical and health sciences Mice 0302 clinical medicine Cancer stem cell Cell Line Tumor Drug Discovery Animals Humans Viability assay Wnt Signaling Pathway 030304 developmental biology Cell Proliferation Pharmacology 0303 health sciences Brain Neoplasms Plant Extracts Wnt signaling pathway Brain Antineoplastic Agents Phytogenic Medicine Korean Traditional Xenograft Model Antitumor Assays Apoptosis 030220 oncology & carcinogenesis Cancer cell Cancer research Neoplastic Stem Cells Stem cell Signal transduction Glioblastoma |
| Zdroj: | Journal of ethnopharmacology. 236 |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacological relevance Korean Red ginseng extract (RG) is one of the most widely used traditional health functional food in Asia, which invigorates immunity and vital energy. RG have been suggested to inhibit proliferation, invasion, and inflammation in several cancer cell lines. Correspondingly, clinical studies have raised the possibility that RG could augment therapeutic efficacy in cancer patients. However, little is known about the anti-cancer effects of RG in glioblastoma (GBM), the most common and aggressive brain tumor for which effective therapeutic regimens need to be developed. Aim of this study: Here, we assessed the in vivo and in vitro anti-cancer properties of RG in a patient-derived xenograft mouse model and GBM stem cell (GSC) line. Materials and methods We evaluated the anti-cancer effects of RG in patient-derived GBM xenograft mice with and without combined concurrent chemo- and radiation therapy (CCRT). Furthermore, we verified the in vitro effects of RG on the proliferation, cell death, and stem cell-like self-renewal capacity of cancer cells. Finally, we investigated the signaling pathway affected by RG, via which its anti-cancer effects were mediated. Results When combined with CCRT, RG impeded GBM progression by reducing cancer cell proliferation and ionized calcium-binding adapter molecule 1 (IBA1)-positive immune cell recruitment. The anti-cancer effects of RG were mediated by Rg3 and Rh2 ginsenosides. Rg3 promoted cell death while Rh2 did not. Furthermore, both Rg3 and Rh2 reduced cell viability and self-renewal capacity of GSCs by inhibiting Wnt/β-catenin signaling. Conclusion Therefore, our observations imply that RG could be applied to the GBM patients in parallel with CCRT to enhance therapeutic efficacy. |
| Databáze: | OpenAIRE |
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