Rat hepatic stellate cells alter the gene expression profile and promote the growth, migration and invasion of hepatocellular carcinoma cells
| Autor: | Zhiming Wang, Binbin Liu, Qin-An Jia, Le-Yuan Zhou, Sheng-Long Ye, Yin-Ying Dong, Yun-Hong Xia |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Cancer Research Carcinoma Hepatocellular Carcinogenicity Tests medicine.medical_treatment Cell Biology Biochemistry matrix metalloprotein-2 -9 Cell Line Paracrine signalling Cell Movement Genetics medicine Hepatic Stellate Cells HSCs Animals Transplantation Homologous Rats Inbred BUF Molecular Biology neoplasms Cell Proliferation Oligonucleotide Array Sequence Analysis cDNA microarray Oncogene Cell growth Hepatocyte Growth Factor Growth factor Liver Neoplasms Articles hepatocellular carcinoma Molecular biology digestive system diseases Coculture Techniques Rats medicine.anatomical_structure Oncology Matrix Metalloproteinase 9 Cell culture Hepatic stellate cell Molecular Medicine Matrix Metalloproteinase 2 Hepatocyte growth factor Female Transcriptome medicine.drug |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 |
| Popis: | The aim of the present study was to examine the effects of activated hepatic stellate cells (HSCs) and their paracrine secretions, on hepatocellular cancer cell growth and gene expression in vitro and in vivo. Differentially expressed genes in McA-RH7777 hepatocellular carcinoma (HCC) cells following non-contact co-culture with activated stellate cells, were identified by a cDNA microarray. The effect of the co-injection of HCC cells and activated HSCs on tumor size in rats was also investigated. Non-contact co-culture altered the expression of 573 HCC genes by >2-fold of the control levels. Among the six selected genes, ELISA revealed increased protein levels of hepatic growth factor, matrix metalloproteinase-2 (MMP-2) and -9 (MMP-9). Incubation of HCC cells with medium conditioned by activated HSCs significantly increased the proliferation rate (P |
| Databáze: | OpenAIRE |
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