Expression of the heparin-binding growth factor midkine and its receptor, Ptprz1, in adult rat pituitary
Autor: | Rita Maliza, Kotaro Horiguchi, Rahimi Syaidah, Dini Ramadhani, Morio Azuma, Motoshi Kikuchi, Alimuddin Tofrizal, Khongorzul Batchuluun, Takehiro Tsukada, Takashi Yashiro, Ken Fujiwara |
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Rok vydání: | 2014 |
Předmět: |
Male
Aging Pituitary gland medicine.medical_specialty Histology In situ hybridization Adrenocorticotropic hormone Pathology and Forensic Medicine Anterior pituitary Internal medicine medicine Animals RNA Messenger Rats Wistar In Situ Hybridization Midkine biology Heparin Receptor-Like Protein Tyrosine Phosphatases Class 5 Cell Biology Molecular biology Growth hormone secretion Prolactin medicine.anatomical_structure Endocrinology Pituitary Gland biology.protein Cytokines Luteinizing hormone |
Zdroj: | Cell and Tissue Research. 359:909-914 |
ISSN: | 1432-0878 0302-766X |
Popis: | Midkine (MK) belongs to a family of secreted heparin-binding growth factors and is highly expressed in various tissues during development. MK has multiple functions, such as regulation of cell proliferation, migration, survival and differentiation. We recently reported that MK mRNA is strongly expressed in the developing rat pituitary gland. In the adult pituitary, however, expression of MK and its receptor and the characteristics of the cells that produce them, have not been determined. Therefore, in this study, we investigate whether MK and its receptor, protein tyrosine phosphatase receptor-type Z (Ptprz1), are present in the adult rat pituitary. In situ hybridization, real-time reverse transcription-PCR and immunoblotting were performed to assess MK and Ptprz1 expression. We also characterize MK- and Ptprz1-expressing cells by double-staining with in situ hybridization and immunohistochemical techniques for each pituitary hormone or S100 protein [a marker of folliculostellate (FS) cells]. MK-expressing cells were located in the anterior and posterior lobes but not in the intermediate lobe. Double-staining and immunoblotting revealed that MK mRNA and protein were only expressed in FS cells in the anterior pituitary. Regarding Ptprz1 expression, Ptprz1 mRNA was detected in adrenocorticotropic hormone (ACTH) cells and growth hormone (GH) cells but not in prolactin cells, thyroid-stimulating hormone cells, luteinizing hormone cells, or FS cells. These findings suggest that MK produced in FS cells acts locally on ACTH cells and GH cells via Ptprz1 in the adult rat anterior pituitary. |
Databáze: | OpenAIRE |
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