Pseudomonas anguilliseptica Strain-A1 Degradation of Polycyclic Aromatic Hydrocarbons in Soil Microcosms: Focus on Detoxification Activity and Free Water-Soluble Protein Extracts Kinetics and Efficiency
| Autor: | Roberto Barone, Edvige Gambino, Marco Trifuoggi, Marco Guida, Maria Toscanesi, Vincenzo Piccialli, Rosa Anna Nastro, Lorenzo De Napoli, Gennaro Picciall |
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| Přispěvatelé: | Barone, Roberto, Nastro, Rosa Anna, Gambino, Edvige, Toscanesi, Maria, Picciall, Gennaro, Napoli, Lorenzo De, Trifuoggi, Marco, Piccialli, Vincenzo, Guida, Marco |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
021110 strategic
defence & security studies Anthracene Chromatography Chemistry Metabolite 0211 other engineering and technologies Soil bioremediation Pseudomonas anquilliseptica-A1 Polycyclic Aromatic Hydrocarbons Enzymatic remediation Ecotoxicology 02 engineering and technology 010501 environmental sciences Phenanthrene Microbial consortium 01 natural sciences High-performance liquid chromatography chemistry.chemical_compound Bioremediation Pyrene Polycyclic Aromatic Hydrocarbon 0105 earth and related environmental sciences Naphthalene |
| Popis: | Pseudomans anguilliseptica-A1 strain, isolated in an urban area, improved the efficiency of a microbial consortium, composed of Bacillaceae, Staphylococcacea, Xantomonadaceae and Enterbacteriaceae, whose ability to degrade five Polycyclic Aromatic Hydrocarbons (PAHs) among the priority pollutants was previously ascertained. Six soil microcosms were prepared with a slurry (60% soil, 40% water) artificially contaminated with anthracene (0.4 mg g-1), phenanthrene (0.2 mg g-1), naphthalene (0.2 mg g-1), pyrene (mg g-1) and benzo(a)pyrene (0.1 mg g-1) and opportunely aerated for two months. PAHs were monthly quantified by inverse phase High Performance Liquid Chromatography (HPLC), coupled with UV-Vis spectrophotometry and spectrofluorimetry. Acute toxicity assays vs Dapnia magna and Lepidium sativum, and chronic essays vs Ceriodaphnia dubia were monthly performed. Our results showed a 100% degradation for naphthalene, 99.14% for anthracene, 99.23% for phenanthrene, 86% for pyrene and 72.5% for benzo[a]pyrene after two months of treatment. A sterile P. anguilliseptica-A1 lysate in Na-K buffer added with each of the chosen PAHs (53%, wtPAHs/volsusp), operated at 30°C the oxidative degradation of naphthalene, pyrene, benzo(a)pyrene and anthracene in a few hours, while the phenanthrene enzyme degradation process took about 15 h. The GC-MS analysis revealed interesting metabolite structures such as 2- hydroxynaphthalene, 9,10-phenanthrenedione, 2,2’ diphenic acid and methyl 4-hydroxybenzoate. The direct utilization of enzymes/microbial extracts from P. anguilliseptica-A1 could present specific advantages such as availability and a fast PAHs degradation time in bioremediation processes. |
| Databáze: | OpenAIRE |
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