Preanalytical study of fetuin-A: effect of sample type, processing speed and freeze–thaw cycles
| Autor: | Naveed Sattar, Lisa McArthur, Paul Welsh, Jennifer Logue, Lisa Johnston |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Serum Adolescent alpha-2-HS-Glycoprotein Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Pilot Projects Plasma Interquartile range Freezing Healthy volunteers Humans Sample Type Centrifugation Edetic Acid Aged Whole blood Chromatography Chemistry Significant difference Temperature General Medicine Middle Aged Fetuin Potential biomarkers Female |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 52:165-168 |
| ISSN: | 1758-1001 0004-5632 |
| DOI: | 10.1177/0004563214529550 |
| Popis: | Background It has been suggested that fetuin-A may be a potential biomarker of cardiometabolic disease. However, few studies have investigated preanalytical factors that might impact the measurement of fetuin-A in the circulation. This pilot study aimed to investigate the preanalytical variables of sample type, timing of sample centrifugation and the impact of freeze–thaw cycles on the concentration of fetuin-A in serum or EDTA-plasma. Methods Blood samples were taken from 19 male or female healthy volunteers, aged 18–70 years, and left at ambient room temperature for 2 h or 48 h. The tubes were then centrifuged, serum and EDTA-plasma separated, and fetuin-A concentrations measured using a commercially available enzyme-linked immunosorbent assay (ELISA). Results There was no significant difference between the concentrations of fetuin-A in EDTA-plasma and serum following separation from whole blood at 2 h postcollection ( P = 0.78). The median (interquartile range) concentrations of fetuin-A in EDTA-plasma separated at 2 h and 48 h postcollection were 589 µg/mL (484–703 µg/mL) and 767 µg/mL (687–942 µg/mL), respectively ( P Conclusion This small pilot study has shown that serum is preferable to EDTA-plasma for the measurement of fetuin-A. It has also shown that fetuin-A appears to be as stable after three freeze–thaw cycles as it is after one. |
| Databáze: | OpenAIRE |
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