Cutibacterium acnes protects Candida albicans from the effect of micafungin in biofilms

Autor: Marion Girardot, Noémie Renaudeau, Christine Imbert, Clément Bernard, Nathalie Quellard, Marie-Laure Mollichella
Přispěvatelé: Ecologie et biologie des interactions (EBI), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Microbiologie de l'Eau (MDE), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS)-Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Service d’Anapathomopathologie, Centre hospitalier universitaire de Poitiers (CHU Poitiers)
Rok vydání: 2018
Předmět:
0301 basic medicine
Microbiology (medical)
Microbiological Techniques
Antifungal Agents
[SDV]Life Sciences [q-bio]
030106 microbiology
Microbiology
Flow cytometry
03 medical and health sciences
chemistry.chemical_compound
Candida albicans
medicine
Humans
Pharmacology (medical)
Propidium iodide
Propionibacterium acnes
ComputingMilieux_MISCELLANEOUS
Microbial Viability
biology
medicine.diagnostic_test
Chemistry
Biofilm
Micafungin
General Medicine
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
biology.organism_classification
Flow Cytometry
In vitro
Corpus albicans
Yeast
Infectious Diseases
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Biofilms
Microscopy
Electron
Scanning
Microbial Interactions
lipids (amino acids
peptides
and proteins)
medicine.drug
Zdroj: International Journal of Antimicrobial Agents
International Journal of Antimicrobial Agents, Elsevier, 2018, 52 (6), pp.942-946. ⟨10.1016/j.ijantimicag.2018.08.009⟩
ISSN: 1872-7913
0924-8579
Popis: The aim of this study was to investigate the ability of Candida albicans and Cutibacterium acnes to grow together as a polymicrobial biofilm in vitro and to examine the influence of C. acnes on C. albicans susceptibility to micafungin. Mature 72-h-old single-species biofilms of C. albicans and polymicrobial biofilms involving both C. albicans and C. acnes were formed in brain-heart infusion and were observed by scanning electronic microscopy. Moreover, 24-h-old single-species and polymicrobial biofilms were treated for 24 h with micafungin (concentrations ranging from 0.75 mg/L to 12 mg/L) and the antibiofilm activity of micafungin was evaluated on fungal cells by flow cytometry following addition of propidium iodide. The results showed that C. albicans and C. acnes formed a polymicrobial biofilm in the tested conditions and that bacterial presence did not modify fungal viability. Micafungin induced a fungal mortality rate ranging from 70-95% in C. albicans single-species biofilms and from 35-40% in C. acnes-C. albicans polymicrobial biofilms. Mortality induced by micafungin was significantly reduced (P < 0.05 for micafungin at 6 mg/L and P < 0.001 for other micafungin concentrations) in polymicrobial conditions compared with single-species biofilms. In conclusion, this study showed that C. albicans and C. acnes are able to form polymicrobial biofilms together in a synergistic way and that this organisation increases yeast resistance to micafungin.
Databáze: OpenAIRE
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