Design of an ultrasound chamber for cellular excitation and observation
| Autor: | Ya Cherng Chu, Yeh Shiu Chu, Jaw-Lin Wang, Cheng Wei Hong, Jormay Lim |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Acoustics and Ultrasonics business.industry Transducers Ultrasound Cell Culture Techniques Acoustics Transducer Energy profile Lamb waves Ultrasonic Waves Arts and Humanities (miscellaneous) Live cell imaging Animals Continuous wave Ultrasonics Mechanosensitive channels Ultrasonic sensor business Cytoskeleton Ultrasonography Biomedical engineering |
| Zdroj: | The Journal of the Acoustical Society of America. 145:EL547-EL553 |
| ISSN: | 0001-4966 |
| Popis: | In this work, a design of integrating ultrasonic transduction with live cell imaging chamber is introduced. The principle of a metal-incident-glass-output acoustic path was used to deliver a uniform energy profile into the imaging/incubation chamber in the form of leaky Lamb waves. The design was applied to examine living mouse mammary gland epithelial cells (EpH4). Significant changes in intracellular activities were observed even at a very low energy intensity level (1 MHz, ISATA = 1 mW/cm2, continuous wave). Live imaging with ultrasonic stimulation provides a different paradigm to interrogate cellular mechanosensitive responses in real time. |
| Databáze: | OpenAIRE |
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