Schizosaccharomyces japonicus has low levels of CoQ10 synthesis, respiration deficiency, and efficient ethanol production
| Autor: | Makoto Kawamukai, Tomohiro Kaino, Shiomi Mochizuki, Kai Tonoko, Yuriko Takashima |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Mutant Applied Microbiology and Biotechnology Biochemistry Cofactor Analytical Chemistry 03 medical and health sciences Respiration Molecular Biology 030102 biochemistry & molecular biology biology Chemistry fungi Organic Chemistry food and beverages General Medicine Fission yeast biology.organism_classification Schizosaccharomyces japonicus Yeast 030104 developmental biology Coenzyme Q – cytochrome c reductase Schizosaccharomyces pombe biology.protein CoQ Fermentation respiration Schizosaccharomyces S. pombe Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 82:1031-1042 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1080/09168451.2017.1401914 |
| Popis: | Coenzyme Q (CoQ) is essential for mitochondrial respiration and as a cofactor for sulfide quinone reductase. Schizosaccharomyces pombe produces a human-type CoQ10. Here, we analyzed CoQ in other fission yeast species. S. cryophilus and S. octosporus produce CoQ9. S. japonicus produces low levels of CoQ10, although all necessary genes for CoQ synthesis have been identified in its genome. We expressed three genes (dps1, dlp1, and ppt1) for CoQ synthesis from S. japonicus in the corresponding S. pombe mutants, and confirmed that they were functional. S. japonicus had very low levels of oxygen consumption and was essentially respiration defective, probably due to mitochondrial dysfunction. S. japonicus grows well on minimal medium during anaerobic culture, indicating that it acquires sufficient energy by fermentation. S. japonicus produces comparable levels of ethanol under both normal and elevated temperature (42 °C) conditions, at which S. pombe is not able to grow. |
| Databáze: | OpenAIRE |
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