Procollagen C-Proteinase Enhancer 1 (PCPE-1) as a Plasma Marker of Muscle and Liver Fibrosis in Mice
| Autor: | Mary Safrin, Eyal Hassoun, Hana Ziv, Sarah Pri-Chen, Efrat Kessler |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology Physiology Respiratory System lcsh:Medicine 030204 cardiovascular system & hematology Platelet membrane glycoprotein Biochemistry Basal (phylogenetics) 0302 clinical medicine Fibrosis Blood plasma Thoracic Diaphragm Medicine and Health Sciences Enzyme-Linked Immunoassays lcsh:Science Multidisciplinary medicine.diagnostic_test Liver Diseases Hematology Animal Models Body Fluids Precipitation Techniques Blood Biomarker (medicine) Liver Fibrosis Anatomy Type I collagen Research Article medicine.medical_specialty Mouse Models Gastroenterology and Hepatology Biology Immunofluorescence Research and Analysis Methods Blood Plasma 03 medical and health sciences Model Organisms medicine Immunoprecipitation Immunoassays lcsh:R Biology and Life Sciences Proteins Correction medicine.disease Procollagen peptidase 030104 developmental biology Immunologic Techniques lcsh:Q Collagens Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 7, p e0159606 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Current non-invasive diagnostic methods of fibrosis are limited in their ability to identify early and intermediate stages of fibrosis and assess the efficacy of therapy. New biomarkers of fibrosis are therefore constantly sought for, leading us to evaluate procollagen C-proteinase enhancer 1 (PCPE-1), a fibrosis-related extracellular matrix glycoprotein, as a plasma marker of fibrosis. A sandwich ELISA that permitted accurate measurements of PCPE-1 concentrations in mouse plasma was established. Tissue fibrosis was assessed using histochemical, immunofluorescence, and immunoblotting analyses for type I collagen and PCPE-1. The normal plasma concentration of PCPE-1 in 6 weeks to 4 months old mice was ~200 ng/ml (189.5 ± 11.3 to 206.8 ± 13.8 ng/ml). PCPE-1 plasma concentrations in four and 8.5 months old mdx mice displaying fibrotic diaphragms increased 27 and 40% respectively relatively to age-matched control mice, an increase comparable to that of the N-propeptide of procollagen type III (PIIINP), a known blood marker of fibrosis. PCPE-1 plasma levels in mice with CCl4-induced liver fibrosis increased 34 to 50% relatively to respective controls and reflected the severity of the disease, namely increased gradually during the progression of fibrosis and went down to basal levels during recovery, in parallel to changes in the liver content of collagen I and PCPE-1. The results favor PCPE-1 as a potential new clinically valuable fibrosis biomarker. |
| Databáze: | OpenAIRE |
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