Species-Related Antigens of Mammalian Cell Strains as Determined by Immunofluorescence
| Autor: | Cyril S. Stulberg, William F. Simpson, Lawrence Berman |
|---|---|
| Rok vydání: | 1961 |
| Předmět: |
biology
medicine.diagnostic_test Cytodiagnosis Cell Fluorescent Antibody Technique Heterologous Cell Biology biology.organism_classification Immunofluorescence Molecular biology General Biochemistry Genetics and Molecular Biology Chinese hamster Staining chemistry.chemical_compound medicine.anatomical_structure Antigen chemistry Allergy and Immunology biology.protein medicine Antigens Antibody Fluorescein isothiocyanate |
| Zdroj: | Experimental Biology and Medicine. 108:434-439 |
| ISSN: | 1535-3699 1535-3702 |
| Popis: | SummaryA procedure has been described whereby species-associated antigens were directly identified in cultured mammalian cells by fluorescent antibody techniques. Guinea pig anti-cellular globulin fractions were conjugated with fluorescein isothiocyanate. Dispersed cells were prepared from monolayer cultures or frozen cell suspensions and the resulting wet cell suspensions were treated with the labeled antibodies. Staining specificity was recognized by presence or absence of peripheral fluorescence of the treated cells. Four cell strains derived from human tissues, 3 from mouse, and one from Chinese hamster, reacted specifically with homologous but not with heterologous labeled antibodies. Antibodies to monkey- and human-derived strains exhibited reciprocal cross-reactions that were eliminated by absorption procedures. |
| Databáze: | OpenAIRE |
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