Marek's disease in broiler chickens: Effect of route of infection and herpesvirus of turkey-vaccination status on detection of virus from blood or spleen by polymerase chain reaction, and on weights of birds, bursa and spleen
| Autor: | Peter J. Groves, Steve W Walkden-Brown, Afm Fakhrul Islam, Susan Burgess |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Marek's disease
animal structures General Immunology and Microbiology viruses animal diseases medicine.medical_treatment Intraperitoneal injection Broiler Spleen Biology biology.organism_classification Virology Virus Vaccination Lymphatic system medicine.anatomical_structure Food Animals hemic and lymphatic diseases Feather visual_art visual_art.visual_art_medium medicine Animal Science and Zoology |
| Zdroj: | Avian Pathology. 30:621-628 |
| ISSN: | 1465-3338 0307-9457 |
| DOI: | 10.1080/03079450120092116 |
| Popis: | The polymerase chain reaction (PCR) has recently emerged as an additional tool for the monitoring and diagnosis of Marek's disease. We investigated a number of factors that may influence the interpretation of PCR results in commercial broiler chickens including the effects of route of infection and herpesvirus of turkeys (HVT)-vaccination status. We also investigated the suitability of peripheral blood lymphocytes (PBL) and spleen as tissues for Marek's disease virus (MDV) detection. HVT-vaccinated and unvaccinated commercial broiler chickens were challenged or not challenged with virulent MDV either by intraperitoneal injection or inhalation of feather dust containing the virus. Blood and spleen samples were collected at weekly intervals to day 35 post-infection for PCR of spleen or PBL. Live weight and lymphoid organ weights were also measured. Spleen and PBL were found to provide similar sensitivity of detection of MDV with a small advantage in favour of spleen. In terms of the timing of detection of MDV, intraperitoneal challenge broadly mimicked natural challenge via inhalation, although infection of birds by inhalation of infective feather dust resulted in slightly later but more complete detection of MDV in challenged birds. Vaccination with HVT delayed the detection of MDV by approximately 10 to 14 days and did not protect against the reduced growth observed in challenged chickens at day 35 post-challenge. |
| Databáze: | OpenAIRE |
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