Rearrangements of the telomeric region of mouse chromosome 11 in Pre-B ABL/MYC cells revealed by mBANDing, spectral karyotyping, and fluorescence in-situ hybridization with a subtelomeric probe
| Autor: | Katalin Benedek, Sabine Mai, Francis Wiener, Ilse Chudoba, George Klein |
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| Rok vydání: | 2004 |
| Předmět: |
Genes
Viral Genes myc Genes abl Biology Transfection medicine.disease_cause Mice hemic and lymphatic diseases Genetics medicine Animals neoplasms In Situ Hybridization Fluorescence B cell Cell Line Transformed Chromosome Aberrations B-Lymphocytes Mice Inbred BALB C ABL medicine.diagnostic_test Chromosome Karyotype Telomere Subtelomere Chromosomes Mammalian Molecular biology Chromosome Banding medicine.anatomical_structure Karyotyping Carcinogenesis Plasmacytoma Fluorescence in situ hybridization |
| Zdroj: | Chromosome Research. 12:777-785 |
| ISSN: | 1573-6849 0967-3849 |
| DOI: | 10.1007/s10577-005-5264-z |
| Popis: | Previous work has demonstrated that chromosome 11 is trisomic or shows a duplicated region encompassing the E1/E2 band of chromosome 11 in 90% of v-abl/myc-induced plasmacytomas (Wiener et al. 1995). In the present report, we have studied BALB/c PreB lymphocytes that were immortalized by v-abl and stably transfected with a conditional MycER vector (Mai et al. 1999). These cells, termed PreB ABL/MYC, showed changes in the E1/E2 bands of chromosome 11 that are similar to those reported previously for v-abl/myc-induced plasmacytomas. This was shown by the use of chromosome painting, SKY, FISH and mBAND. Our findings suggest that the Pre-B ABL/MYC cells may be used to analyse the genetic changes affecting chromosome 11 that are associated with v-abl/myc-dependent tumorigenesis in mouse B cells. |
| Databáze: | OpenAIRE |
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