The role of circulating miRNAs and CA19-9 in pancreatic cancer diagnosis
| Autor: | Mucio Luiz de Assis Cirino, Luciano Neder Serafini, Alberto Facury Gaspar, Daniela Pretti da Cunha Tirapelli, Fermino Sanches Lizarte Neto, Jorge Resende Lopes Júnior, José Celso Ardengh, Nivaldo Faria Vieira, Fabiano Pinto Saggioro, José Sebastião dos Santos, Paulo Novais, Rafael Kemp, Ajith Kumar Sankarankutty |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Circulating mirnas
medicine.medical_specialty Pancreatic ductal adenocarcinoma microRNA Response to therapy business.industry MICRORNAS pancreatic cancer biomarkers CA19-9 medicine.disease Gastroenterology Clinical Practice cancer recurrence Oncology Internal medicine Pancreatic cancer Healthy volunteers medicine business Research Paper |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Oncotarget |
| Popis: | Diagnosis and treatment of pancreatic ductal adenocarcinoma (PA) remains a challenge in clinical practice. The aim of this study was to assess the role of microRNAs (miRNAs-21, -23a, -100, -107, -181c, -210) in plasma and tissue as possible biomarkers in the diagnosis of PA. Samples of plasma (PAp-n = 13), pancreatic tumors (PAt-n = 18), peritumoral regions (PPT-n = 9) were collected from patients during the surgical procedure. The control group consisted of samples from patients submitted to pancreatic surgery for trauma or cadaveric organs (PC-n = 7) and healthy volunteers donated blood (PCp-n = 6). The expression profile of microRNAs was measured in all groups using RT-PCR, serum CA19-9 levels were determined in PA and PC. In tissue samples, there was a difference in the expression of miRNAs-21, -210 (p < 0.05) across the PAt, PC and PPT groups. The PAp showed overexpression of miRNAs-181c, -210 (p < 0.05) when compared to PCp. The combination of miRNAs-21, -210 tissue expression and serum CA19-9 showed 100% accuracy in the diagnosis of PA, as well as miR-181c expression in the plasma (PApxPCp). The expression of microRNAs in plasma proved to be a promising tool for a noninvasive detection test for PA, as well as further studies will evaluate the utility of microRNAs expression as biomarkers for prognostic and response to therapy in PA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|