Assessment of concentration and penetration depth of cisplatin in human lung tissue after decortication and hyperthermic exposure
| Autor: | Elena Loch, Christopher Larisch, Karolina Mueller, Patrick J. Bednarski, Michael Ried, Christian Großer, Till Markowiak, Hans-Stefan Hofmann |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cisplatin
medicine.medical_specialty business.industry Hyperthermic perfusion medicine.medical_treatment Urology General Medicine Decortication Pleural cavity Human lung Original Article on Hyperthermic Intraoperative Chemotherapy (HITHOC) in Thoracic Surgical Oncology medicine.anatomical_structure Medicine business Lung tissue Penetration depth Pleurectomy medicine.drug |
| Zdroj: | Ann Transl Med |
| ISSN: | 2305-5847 2305-5839 |
| DOI: | 10.21037/atm-20-6307 |
| Popis: | BACKGROUND: Hyperthermic perfusion of the pleural cavity with cisplatin after pleurectomy/decortication is an additional therapeutic option to reduce local relapse of malignant pleural tumours. Although there are data on the clinical effect, only little is known about the local impact on human lung tissue by cisplatin. The objective of this experimental study is to evaluate both the concentration and the penetration depth of cisplatin in human lung tissue after normothermic and hyperthermic exposure under ex-vivo-in-vitro conditions. METHODS: This study was approved by the local ethics committee. In total, 46 patients underwent elective lobectomy and wedge resections were taken from the resected lobes. A decortication of the visceral pleura was performed under ex-vivo conditions, and the tissue samples were incubated with cisplatin (c =0.05 mg/mL) at 37, 42 or 45 °C for 60 minutes. Then the mass concentration of platinum was measured with flameless atomic absorption spectroscopy and then converted into cisplatin concentration. In addition, the current data were compared with previous data of our working group (42 °C, without decortication). RESULTS: The overall maximum penetration depth was 7.5 mm due to limitations of our methods. The functional maximum penetration depth did not vary with temperature (P=0.243) but by decortication (P |
| Databáze: | OpenAIRE |
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