Antioxidant activity, anti-proliferative activity, and amino acid profiles of ethanolic extracts of edible mushrooms
| Autor: | S Panthong, N Boonsathorn, Siriporn Chuchawankul |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antioxidant medicine.medical_treatment Trolox equivalent antioxidant capacity 01 natural sciences Antioxidants 03 medical and health sciences chemistry.chemical_compound Inhibitory Concentration 50 Phenols 010608 biotechnology Lactate dehydrogenase Genetics medicine Potency Humans Metabolomics Viability assay Food science Amino Acids Cytotoxicity Molecular Biology Chromatography High Pressure Liquid Cell Proliferation Flavonoids Mushroom Pleurotus 030102 biochemistry & molecular biology biology Cell Death Ethanol General Medicine U937 Cells biology.organism_classification chemistry Camptothecin Agaricales |
| Zdroj: | Genetics and molecular research : GMR. 15(4) |
| ISSN: | 1676-5680 |
| Popis: | Biological activities of various mushrooms have recently been discovered, particularly, immunomodulatory and antitumor activities. Herein, three edible mushrooms, Auricularia auricula-judae (AA), Pleurotus abalonus (PA) and Pleurotus sajor-caju (PS) extracted using Soxhlet ethanol extraction were evaluated for their antioxidative, anti-proliferative effects on leukemia cells. Using the Folin-Ciocalteau method and Trolox equivalent antioxidant capacity assay, phenolics and antioxidant activity were found in all sample mushrooms. Additionally, anti-proliferative activity of mushroom extracts against U937 leukemia cells was determined using a viability assay based on mitochondrial activity. PA (0.5 mg/mL) and AA (0.25-0.5 mg/mL) significantly reduced cell viability. Interestingly, PS caused a hormetic-like biphasic dose-response. Low doses (0-0.25 mg/L) of PS promoted cell proliferation up to 140% relative to control, whereas higher doses (0.50 mg/mL) inhibited cell proliferation. Against U937 cells, AA IC50 was 0.28 ± 0.04 mg/mL, which was lower than PS or PA IC50 (0.45 ± 0.01 and 0.49 ± 0.001 mg/mL, respectively). Furthermore, lactate dehydrogenase (LDH) leakage conferred cytotoxicity. PS and PA were not toxic to U937 cells at any tested concentration; AA (0.50 mg/mL) showed high LDH levels and caused 50% cytotoxicity. Additionally, UPLC-HRMS data indicated several phytochemicals known to support functional activities as either antioxidant or anti-proliferative. Glutamic acid was uniquely found in ethanolic extracts of AA, and was considered an anti-cancer amino acid with potent anti-proliferative effects on U937 cells. Collectively, all mushroom extracts exhibited antioxidant effects, but their anti-proliferative effects were dose-dependent. Nevertheless, the AA extract, with highest potency, is a promising candidate for future applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|