Investigating the impact of metal ions and 3D printed droplet microfluidics chip geometry on the luminol‑potassium periodate chemiluminescence system for estimating total phenolic content in olive oil
| Autor: | Fakhr Eldin O. Suliman, Baqia Al Mughairy, Haider A.J. Al-Lawati |
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| Rok vydání: | 2019 |
| Předmět: |
Potassium Compounds
Metal ions in aqueous solution Microfluidics Analytical chemistry 02 engineering and technology 010402 general chemistry 01 natural sciences Analytical Chemistry Luminol law.invention Ion chemistry.chemical_compound Phenols law Lab-On-A-Chip Devices Olive Oil Instrumentation Spectroscopy Chemiluminescence Quenching (fluorescence) Periodic Acid Periodate Equipment Design 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics 0104 chemical sciences Potassium periodate chemistry Metals Luminescent Measurements Printing Three-Dimensional 0210 nano-technology Food Analysis |
| Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 221:117182 |
| ISSN: | 1386-1425 |
| DOI: | 10.1016/j.saa.2019.117182 |
| Popis: | The impact of the chip design and the mixing mechanisms using six different 3D printed microfluidic chips were investigated. The study was conducted using novel 3D printed droplet based microfluidics. A multi-mixing approach was utilized to enhance the CL signal of the CL system under investigation. The approach is based on droplet formation, droplet mixing and droplets merging in the 3D printed microfluidic chip. A 154% higher CL signal intensity was obtained using this approach compared to the CL signal obtained using the serpentine chip commonly used for improving the mixing inside droplet microfluidics. This chip was exploited to study the role of three metal ions: Co2+, Mn2+ and Fe2+ on catalyzing the luminol‑potassium periodate chemiluminescence (CL) reaction with selected phenolic compounds in basic media was carefully investigated. Furthermore, the luminol‑potassium periodate-metal ions system was optimized for all metal ions using gallic acid as the reference standard. Despite the popularity of luminol systems in estimating antioxidant activity or total phenolic content (TPC), the results of this study revealed the necessity of careful and vigilant attention when applying it to complex matrices. The only metal ion that showed quenching behavior with all 20 of the tested phenolic compounds was Fe2+, while Co2+and Mn2+ showed both quenching and enhancement in the CL signal. The luminol‑potassium periodate-Fe2+ system was applied to estimate TPC in olive oil extracts. |
| Databáze: | OpenAIRE |
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