Structure-guided synthesis of a protein-based fluorescent sensor for alkyl halides
| Autor: | Hakbong Lee, Jeong Kon Seo, Yerkin Dunbayev, Myeong-Gyun Kang, Changwook Lee, Hyun-Woo Rhee, Beom Ho Kim |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
010405 organic chemistry Chemistry Metals and Alloys Halide General Chemistry 010402 general chemistry Ligand (biochemistry) Photochemistry 01 natural sciences Fluorescence Combinatorial chemistry Catalysis Receptor–ligand kinetics 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Covalent bond Hydrolase Materials Chemistry Ceramics and Composites Protein alkylation Alkyl |
| Zdroj: | Chemical Communications. 53:9226-9229 |
| ISSN: | 1364-548X 1359-7345 |
| DOI: | 10.1039/c7cc03714g |
| Popis: | Alkyl halides are potentially mutagenic carcinogens. However, no efficient fluorescent sensor for alkyl halide detection in human-derived samples has been developed to date. Herein, we report a new protein-based fluorescent sensor for alkyl halides. Analysis of the HaloTag holo-crystal structure with its covalently attached ligand revealed an unexpected cavity, allowing for the design of a new fluorogenic ligand. This ligand showed the highest fluorescence response (300-fold) and fastest binding kinetics (t1/2 < 150 s) to a HaloTag mutant (M175P) protein. This protein-based sensor system was effectively used to detect alkyl halides in human serum and monitor real-time protein alkylation. |
| Databáze: | OpenAIRE |
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