Mouse Embryo Cryopreservation by Slow Freezing
| Autor: | Robert Taft |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Cryopreservation
0301 basic medicine Slow freezing animal structures Embryo Biology Embryo Mammalian Insemination General Biochemistry Genetics and Molecular Biology Andrology Mice 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Embryo cryopreservation Freezing embryonic structures medicine Animals Female Blastocyst 030217 neurology & neurosurgery |
| Zdroj: | Cold Spring Harbor Protocols. 2018:pdb.prot094540 |
| ISSN: | 1559-6095 1940-3402 |
| DOI: | 10.1101/pdb.prot094540 |
| Popis: | This embryo cryopreservation protocol is used by The Jackson Laboratory and several other repositories around the world. This protocol has clearly withstood the test of time; >2 million embryos from thousands of strains have been cryopreserved using this method. It requires a controlled-rate freezer, but is simple, effective, and can be applied to cryopreserve embryos from the two-cell to the blastocyst stage. Thawing requires neither special media nor equipment because the insemination straw contains all of the needed elements, making this very useful for distributing cryopreserved embryos. Embryo survival rates of greater than 90% can be expected when this method is used, making it reliable and enabling staff with prior embryo-handling experience to become proficient quickly. |
| Databáze: | OpenAIRE |
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