| Autor: |
A. Chabronova, G.G.H. van den Akker, B.A.C. Housmans, M.M.J. Caron, A. Cremers, D.A.M. Surtel, K. Wichapong, M.M.J. Peffers, L.W. van Rhijn, V. Marchand, Y. Motorin, T.J.M. Welting |
| Přispěvatelé: |
Orthopedie, RS: CAPHRI - R3 - Functioning, Participating and Rehabilitation, RS: CAPHRI School for Public Health and Primary Care, MUMC+: MA Orthopedie (9), Biochemie, RS: Carim - B01 Blood proteins & engineering |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Osteoarthritis and Cartilage, 31(3), 374-385. ELSEVIER SCI LTD |
| ISSN: |
1063-4584 |
| Popis: |
Objective: Osteoarthritis-related cartilage extracellular matrix remodeling is dependent on changes in chondrocyte protein expression. Yet, the role of ribosomes in chondrocyte translation regulation is un-known. In this exploratory study, we investigated ribosomal RNA (rRNA) epitranscriptomic-based ribo-some heterogeneity in human articular chondrocytes and its relevance for osteoarthritis.Methods: Sequencing-based rRNA 2'-O-methylation profiling analysis (RiboMethSeq) was performed on non-OA primary human articular chondrocytes (n = 5) exposed for 14 days to osteoarthritic synovial fluid (14 donors, pooled, 20% v/v). The SW1353 SNORD71 KO cell pool was generated using Lenti-CRISPRv2/Cas9. The mode of translation initiation and fidelity were determined by dual-luciferase re-porters. The cellular proteome was analyzed by LC-MS/MS and collagen type I protein expression was evaluated by immunoblotting. Loading of COL1A1 mRNA into polysomes was determined by sucrose gradient ultracentrifugation and fractionation.Results: We discovered that osteoarthritic synovial fluid instigates site-specific changes in the rRNA 2'-O-me profile of primary human articular chondrocytes. We identified five sites with differential 2'-O-me levels. The 2'-O-me status of 5.8S-U14 (one of identified differential 2'-O-me sites; decreased by 7.7%, 95% CI [0.9-14.5%]) was targeted by depleting the level of its guide snoRNA SNORD71 (50% decrease, 95% CI [33-64%]). This resulted in an altered ribosome translation modus (e.g., CrPV IRES, FC 3, 95% CI [2.2-4.1]) and promoted translation of COL1A1 mRNA which led to increased levels of COL1A1 protein (FC 1.7, 95% CI [1.3-2.0]).Conclusions: Our data identify a novel concept suggesting that articular chondrocytes employ rRNA epitranscriptomic mechanisms in osteoarthritis development.(c) 2023 The Authors. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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