Immunoaffinity Resin for Purification of Urinary Leukotriene E4
| Autor: | Kirk M Maxey, Sally E. Wenzel, James MacDonald, Jay Y. Westcott |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
Leukotriene synthesis Physiology Coefficient of variation Urinary system Urine Biochemistry High-performance liquid chromatography Chromatography Affinity Immunoenzyme Techniques chemistry.chemical_compound Urinary levels Humans Child Leukotriene E4 Pharmacology Creatinine Chromatography Antibodies Monoclonal Reproducibility of Results Cell Biology Middle Aged respiratory system chemistry Child Preschool Hemocyanins lipids (amino acids peptides and proteins) Immunosorbents |
| Zdroj: | Prostaglandins & Other Lipid Mediators. 55:301-321 |
| ISSN: | 1098-8823 |
| DOI: | 10.1016/s0090-6980(98)00027-6 |
| Popis: | Urinary leukotriene E4 (LTE4) has been used as an index of total leukotriene synthesis. A wide variety of methods have been applied to measure LTE4 which has made direct comparison of urinary levels reported by different laboratories difficult. A new peptidoleukotriene immunoaffinity resin was utilized for urinary LTE4 purification in a method that is easy and inexpensive, utilizing commercially available reagents. This method is described and compared to other methods. LTE4 (50–250 pg/mL) added to a urine extract was quantitatively recovered using the immunoaffinity resin. Similarly, LTE4 (50–400 pg/mL) added to urine was recovered between 63 and 76%. The coefficient of variation of samples purified and quantified on the same or on different days ranged from 8–10%. There was a strong correlation (r2 = 0.95) between LTE4 concentrations determined after immunofiltration and immunoaffinity purification. Although there was a good correlation between urinary LTE4 levels measured without purification compared to after immunoaffinity purification, the high y-intercept of 179 indicates the presence of interfering substances in unpurified urine. Urinary LTE4 in normal healthy adults was 80 ± 7 pg/mg creatinine, similar to that previously reported following HPLC or immunofiltration purification. Urinary LTE4 was also measured in healthy children (age 3–12) and found to be 103 ± 9. |
| Databáze: | OpenAIRE |
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