Influence of routes and administration parameters on antibody response of pigs following DNA vaccination
| Autor: | B. Viuff, Tove Dannemann-Jensen, Merete Blixenkrone Møller, Anette Bøtner, Søren Kamstrup, Annette Malene Barfoed, Birte Kristensen |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Injections
Intradermal Swine Enzyme-Linked Immunosorbent Assay Biology Antibodies Viral Injections Intramuscular Gene gun DNA vaccination Mice Antigen Vaccines DNA Animals Porcine respiratory and reproductive syndrome virus Particle Size DNA Primers Mucous Membrane General Veterinary General Immunology and Microbiology Viral Vaccine Vaccination Public Health Environmental and Occupational Health Viral Vaccines DNA Biolistics Porcine reproductive and respiratory syndrome virus biology.organism_classification Virology Nucleoproteins Infectious Diseases Humoral immunity Immunology Molecular Medicine Gold Plasmids |
| Zdroj: | Vaccine. 22:1395-1405 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2003.10.032 |
| Popis: | Using the nucleoprotein of porcine reproductive and respiratory syndrome virus as model antigen, we optimised parameters for gene gun vaccination of pigs, including firing pressure and vaccination site. As criteria for optimisation, we characterised particle penetration and local tissue damage by histology. For selected combinations, vaccination efficiency in terms of antibody response was studied. Gene gun vaccination on ear alone was as efficient as a multi-site (ear, thorax, inguinal area, tongue mucosa) gene gun approach, and more efficient than combined intramuscular (i.m.)/intradermal (i.d.) injection of plasmid DNA. This indicates, that the ear is an attractive site for gene gun vaccination of pigs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect