A method for chronic membrane plasmapheresis in the rat
| Autor: | T. J. Nasca, H. H. Euler, A. J. Pinevich, D. B. Follette, D. Kerr |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Physiology medicine.medical_treatment Ultrafiltration Pharmacology Inflammatory bowel disease Immunoglobulin G Rats Sprague-Dawley In vivo Physiology (medical) Diabetes mellitus medicine Animals Rats Wistar Autoimmune disease biology business.industry Membranes Artificial Plasmapheresis medicine.disease Acute toxicity Rats Apheresis Immunoglobulin M Immunology Blood Component Removal biology.protein Female business |
| Zdroj: | Journal of Applied Physiology. 75:2820-2824 |
| ISSN: | 1522-1601 8750-7587 |
| DOI: | 10.1152/jappl.1993.75.6.2820 |
| Popis: | Therapeutic apheresis as applied to humans may encompass a single treatment or numerous treatments for disorders ranging from acute poisoning to severe chronic autoimmune disease. However, the mechanisms of beneficial effects of apheresis are not well characterized. Utilizing a miniaturized hollow-fiber membrane system, we have developed a reliable technique for long-term vascular access in the rat that permits repetitive plasmapheresis. We established vascular access in 14 animals, with 8 and 6 rats randomized to 3- and 7-wk experimental periods, respectively. Immunoglobulin levels of blood samples obtained immediately before and after each plasmapheresis were measured to examine membrane filtration characteristics. Overall, 100% of the animals survived and 93% successfully completed their assigned experimental periods. Mean decrease of immunoglobulin G and M levels for 28 plasmapheresis treatments in five rats was 66.9 +/- 8.1 and 61.0 +/- 7.3% (SD), respectively, indicating effective membrane filtration. This model of apheresis can be applied to several disorders in the rat, including, but not limited to, spontaneous insulin-dependent diabetes mellitus and experimental inflammatory bowel disease. |
| Databáze: | OpenAIRE |
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