Decay-accelerating factor (DAF/CD55) is a functional active element of the LPS receptor complex
| Autor: | A. Lentschat, Artur J. Ulmer, Holger Heine, Volker T. El-Samalouti, Lutz Hamann |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
0301 basic medicine
Chinese hamster ovary cell 030106 microbiology Immunology Cell Stimulation Cell Biology Transfection Biology Microbiology Complement system Cell biology Lipid A 03 medical and health sciences 0302 clinical medicine Infectious Diseases medicine.anatomical_structure Membrane protein medicine lipids (amino acids peptides and proteins) Molecular Biology Decay-accelerating factor 030215 immunology |
| Zdroj: | Journal of Endotoxin Research. 7:227-231 |
| ISSN: | 0968-0519 |
| Popis: | Previously, we identified an 80 kDa membrane protein (LMP80) that is capable of binding to LPS and lipid A in the presence of LBP and sCD14. LMP80 could also be detected after immuno-coprecipitation of cell membranes with LPS and lipid A, indicating a physical contact of LMP80 and LPS/lipid A. Further analysis and peptide sequencing revealed that LMP80 is identical to CD55 (decay accelerating factor, DAF), a regulatory molecule of the complement cascade. Transfection of LPS-hyporesponsive Chinese hamster ovary (CHO) cells with human CD55 resulted in the translocation of NF-κB upon stimulation with LPS or lipid A. Our results demonstrate a new functional role of CD55 as a molecule able to mediate LPS-induced activation of cells that may be part of a multimeric LPS receptor complex. |
| Databáze: | OpenAIRE |
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