Modulation of Dendritic Cell Apoptosis and CD8+ Cytotoxicity by Histamine: Role of Protein Kinase C
| Autor: | Enrique Podaza, Mónica Vermeulen, Gabriela Salamone, Julieta María Alcain, María Soledad Gori |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
CIENCIAS MÉDICAS Y DE LA SALUD Article Subject Immunology Blotting Western Inmunología Apoptosis CD8-Positive T-Lymphocytes Major histocompatibility complex DENDRITIC CELLS 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine HISTAMINE lcsh:Pathology Cytotoxic T cell Animals Receptor Cytotoxicity Protein kinase C Cells Cultured Protein Kinase C Microscopy Confocal biology Cell Biology purl.org/becyt/ford/3.1 [https] CYTOTOXIC T LYMPHOCYTES Dendritic Cells Flow Cytometry Molecular biology Cell biology Mice Inbred C57BL PROTEIN KINASE C Medicina Básica 030104 developmental biology chemistry biology.protein purl.org/becyt/ford/3 [https] Female Histamine CD8 lcsh:RB1-214 030215 immunology Research Article |
| Zdroj: | Mediators of Inflammation CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Mediators of Inflammation, Vol 2017 (2017) |
| ISSN: | 1466-1861 0962-9351 |
| Popis: | Dendritic cells (DC) are able to present extracellular antigens associated with the molecules of the major histocompatibility complex class I. In a previous work, we demonstrated that the histamine (HIS), acting through H1/H4 receptors, increases the cross-presentation of soluble ovalbumin by murine DC and can to enhance the recruitment of specific CD8+ T lymphocytes during the development of chronic inflammatory responses. Here, we studied in more depth the mechanisms underlying this enhancement. We showed that the cytotoxicity of specific CD8+ lymphocytes is increased in HIS-treated DC and it is lost by inhibition of vacuolar-ATPase that prevents endosome acidification. It is known that HIS acts through G-protein coupled receptors. The H1/H4 receptors are associated with a Gq subunit, which involves PKC signaling, a pathway related to the apoptotic process. Interestingly, we demonstrated for the first time that HIS prevents DC apoptosis induced by heat-shock through the inhibition of caspase-3, a mechanismdependent on PKC activation, since it is reversed by its inhibition. By contrast, cytolytic activity of T lymphocytes induced by HIS-stimulated DC was independent of PKC pathway. Fil: Alcain, Julieta María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Podaza, Enrique Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Gori, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Salamone, Gabriela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina |
| Databáze: | OpenAIRE |
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