Plant age and genotype affect the bacterial community composition in the tuber rhizosphere of field-grown sweet potato plants
Autor: | Guo-Chun Ding, Lucy Seldin, Kornelia Smalla, Thais Freitas da Silva, Joana Montezano Marques, Arie Fitzgerald Blank, Renata Estebanez Vollú |
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Rok vydání: | 2014 |
Předmět: |
Rhizosphere
Bacteria Genotype Ecology biology Denaturing Gradient Gel Electrophoresis Starch fungi Pseudomonas Bulk soil food and beverages Chryseobacterium biology.organism_classification Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound chemistry RNA Ribosomal 16S Botany Pyrosequencing Stenotrophomonas Ipomoea batatas Soil Microbiology Temperature gradient gel electrophoresis |
Zdroj: | FEMS Microbiology Ecology. 88:424-435 |
ISSN: | 0168-6496 |
Popis: | The hypothesis that sweet potato genotypes containing different starch yields in their tuberous roots can affect the bacterial communities present in the rhizosphere (soil adhering to tubers) was tested in this study. Tuberous roots of field-grown sweet potato of genotypes IPB-149 (commercial genotype), IPB-052, and IPB-137 were sampled three and six months after planting and analyzed by denaturing gradient gel electrophoresis (DGGE) and pyrosequencing analysis of 16S rRNA genes PCR-amplified from total community DNA. The statistical analysis of the DGGE fingerprints showed that both plant age and genotypes influenced the bacterial community structure in the tuber rhizosphere. Pyrosequencing analysis showed that the IPB-149 and IPB-052 (both with high starch content) displayed similar bacterial composition in the tuber rhizosphere, while IPB-137 with the lowest starch content was distinct. In comparison with bulk soil, higher 16S rRNA gene copy numbers (qPCR) and numerous genera with significantly increased abundance in the tuber rhizosphere of IPB-137 (Sphingobium, Pseudomonas, Acinetobacter, Stenotrophomonas, Chryseobacterium) indicated a stronger rhizosphere effect. The genus Bacillus was strongly enriched in the tuber rhizosphere samples of all sweet potato genotypes studied, while other genera showed a plant genotype-dependent abundance. This is the first report on the molecular identification of bacteria being associated with the tuber rhizosphere of different sweet potato genotypes. |
Databáze: | OpenAIRE |
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