Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex-sorted bull sperm by inhibiting the oxidative stress
| Autor: | Chong-Yang Li, Pei-Pei Zhang, Huiying Zou, Hao-Yu Wang, Hai-Sheng Hao, Yun-Wei Pang, Hua-Bin Zhu, Wei-Hua Du, Peng-Cheng Jiao, Shan-Jiang Zhao, Sha Yang, Jing-Jing Wang, Tong Hao, Jinming Huang, Xue-Ming Zhao, Wei-Min Ruan |
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| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_treatment Ascorbic Acid Fertilization in Vitro Phosphatidylserines Membrane Potentials Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Human fertilization Lycopene Malondialdehyde medicine Animals Blastocyst Sex Preselection Incubation reproductive and urinary physiology 030219 obstetrics & reproductive medicine In vitro fertilisation Vitamin C urogenital system 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science Sperm Spermatozoa medicine.anatomical_structure chemistry Animal Science and Zoology Cattle Biotechnology |
| Zdroj: | Reproduction in domestic animals = ZuchthygieneREFERENCES. 55(9) |
| ISSN: | 1439-0531 |
| Popis: | The fertilization capacity of sex-sorted sperms is seriously decreased, which inhibits its wide application. However, little information is still available about the effect of vitamin C (VC) and lycopene (Lyc) on the fertilization capacity of sex-sorted bull sperm. In this study, the washing medium and fertilization medium of sex-sorted sperm from three bull individuals were supplemented with different concentrations of VC (0, 1 × 10-3 , 1 × 10-4 , 1 × 10-5 , 1 × 10-6 M) or Lyc (0, 1 × 10-4 , 1 × 10-5 , 1 × 10-6 , 1 × 10-7 ). After washing twice and incubation for 1.5 hr, the malondialdehyde (MDA) level, phosphatidylserine (PS) translocation, membrane potential (Δψm) and IVF (in vitro fertilization) ability of sex-sorted sperm were investigated. For the sex-sorted sperm of bulls A, B and C, 1 × 10-3 M VC or 1 × 10-4 M Lyc treatment significantly decreased their MDA levels and PS translocation and increased their Δψm levels and cleavage rates after IVF. When blastocysts were concerned, 1 × 10-4 M Lyc significantly improved the blastocyst rates and their IFN-tau expression of bulls A and C. In conclusion, supplementation of 1 × 10-3 M VC or 1 × 10-4 M Lyc in washing and fertilization medium contributed greatly to improving the fertilization capacity of sex-sorted bull sperm during IVF procedure. |
| Databáze: | OpenAIRE |
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