BST2 Mediates Osteoblast Differentiation via the BMP2 Signaling Pathway in Human Alveolar-Derived Bone Marrow Stromal Cells
| Autor: | Jun Lee, Hong-In Shin, Eui-Sic Cho, Beom-Su Kim, Jae Goo Kim, Hyung-Keun You, Hyun-Dae Lim, Su-Hyang Yoo, Sung-Hee Pi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Bone sialoprotein Cell signaling Cellular differentiation Protein Expression lcsh:Medicine Bone Morphogenetic Protein 2 Artificial Gene Amplification and Extension Signal transduction Biochemistry Polymerase Chain Reaction 0302 clinical medicine Animal Cells Osteogenesis Medicine and Health Sciences Small interfering RNAs RNA Small Interfering lcsh:Science Cells Cultured Connective Tissue Cells Gene knockdown Multidisciplinary biology Chemistry Osteoblast Cell Differentiation Middle Aged Osteoblast Differentiation Cell biology Nucleic acids medicine.anatomical_structure Connective Tissue 030220 oncology & carcinogenesis embryonic structures Cellular Types Anatomy Research Article Stromal cell animal structures SMAD signaling Bone Marrow Cells Research and Analysis Methods GPI-Linked Proteins Bone morphogenetic protein 2 03 medical and health sciences Antigens CD medicine Genetics Gene Expression and Vector Techniques Humans Non-coding RNA Molecular Biology Techniques Molecular Biology Molecular Biology Assays and Analysis Techniques Osteoblasts Mesenchymal stem cell lcsh:R Biology and Life Sciences Mesenchymal Stem Cells Cell Biology Molecular biology Gene regulation 030104 developmental biology Biological Tissue Gene Expression Regulation biology.protein RNA lcsh:Q Bone marrow Gene expression Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 6, p e0158481 (2016) PLOS ONE(11): 6 |
| ISSN: | 1932-6203 |
| Popis: | The molecular mechanisms controlling the differentiation of bone marrow stromal stem cells into osteoblasts remain largely unknown. In this study, we investigated whether bone marrow stromal antigen 2 (BST2) influences differentiation toward the osteoblasts lineage. BST2 mRNA expression in human alveolar-derived bone marrow stromal cells (hAD-BMSCs) increased during differentiation into osteoblasts. hAD-BMSCs differentiation into osteoblasts and the mRNA expression of the bone-specific markers alkaline phosphatase, collagen type α 1, bone sialoprotein, osteocalcin, and osterix were reduced by BST2 knockdown using siRNA. Furthermore, BST2 knockdown in hAD-BMSCs resulted in decreased RUNX2 mRNA and protein expression. We hypothesized that BST2 is involved in differentiation of into osteoblasts via the BMP2 signaling pathway. Accordingly, we evaluated the mRNA expression levels of BMP2, BMP receptors (BMPR1 and 2), and the downstream signaling molecules SMAD1, SMAD4, and p-SMAD1/5/8 in BST2 knockdown cells. BMP2 expression following the induction of differentiation was significantly lower in BST2 knockdown cells than in cells treated with a non-targeting control siRNA. Similar results were found for the knockdown of the BMP2 receptor- BMPR1A. We also identified significantly lower expression of SMAD1, SMAD4, and p-SMAD1/5/8 in the BST2 knockdown cells than control cells. Our data provide the first evidence that BST2 is involved in the osteogenic differentiation of bone marrow stromal cells via the regulation of the BMP2 signaling pathway. |
| Databáze: | OpenAIRE |
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