A Role for the αvβ3 Integrin in the Transmigration of Monocytes
| Autor: | Eric J. Brown, Beat A. Imhof, Dheepika Weerasinghe, Frederick P. Ross, Kevin P. McHugh, Roland H. Gisler |
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| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
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Complementary ICAM-1 Intercellular Adhesion Molecule-1 Integrin Platelet Membrane Glycoproteins In Vitro Techniques migration Transfection CD49c Monocytes Collagen receptor Cell Line 03 medical and health sciences Mice L Cells Antigens CD Cell Movement Cell Adhesion Animals Humans Receptors Vitronectin Endothelium Cell adhesion 030304 developmental biology 0303 health sciences Integrin alphaVbeta3 αLβ2 integrin biology αvβ3 integrin 030302 biochemistry & molecular biology Integrin beta3 Cell Biology Articles Lymphocyte Function-Associated Antigen-1 Recombinant Proteins Cell biology Integrin alpha M embryonic structures monocyte cardiovascular system biology.protein Integrin beta 6 biological phenomena cell phenomena and immunity |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | The beta2 integrins and intercellular adhesion molecule-1 (ICAM-1) are important for monocyte migration through inflammatory endothelium. Here we demonstrate that the integrin alphavbeta3 is also a key player in this process. In an in vitro transendothelial migration assay, monocytes lacking beta3 integrins revealed weak migratory ability, whereas monocytes expressing beta3 integrins engaged in stronger migration. This migration could be partially blocked by antibodies against the integrin chains alphaL, beta2, alphav, or IAP, a protein functionally associated with alphavbeta3 integrin. Transfection of beta3 integrin chain cDNA into monocytes lacking beta3 integrins resulted in expression of the alphavbeta3 integrin and conferred on these cells an enhanced ability to transmigrate through cell monolayers expressing ICAM-1. These monocytes also engaged in alphaLbeta2-dependent locomotion on recombinant ICAM-1 which was enhanced by alphavbeta3 integrin occupancy. Antibodies against IAP were able to revert this alphavbeta3 integrin-dependent cell locomotion to control levels. Finally, adhesion assays revealed that occupancy of alphavbeta3 integrin could decrease monocyte binding to ICAM-1. In conclusion, we show that alphavbeta3 integrin modulates alphaLbeta2 integrin-dependent monocyte adhesion to and migration on ICAM-1. This could represent a novel mechanism to promote monocyte motility on vascular ICAM-1 and initiate subsequent transendothelial migration. |
| Databáze: | OpenAIRE |
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