Shigella flexneri type-specific antigen V: cloning, sequencing and characterization of the glucosyl transferase gene of temperate bacteriophage SfV
| Autor: | Alf A. Lindberg, Pham Thi Huan, Belinda Whittle, Naresh K. Verma, David A Bastin |
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| Rok vydání: | 1997 |
| Předmět: |
Sequence analysis
viruses Molecular Sequence Data Gene Expression Homology (biology) Shigella flexneri Bacteriophage Open Reading Frames Transformation Genetic Lysogenic cycle Genetics Bacteriophages Amino Acid Sequence Cloning Molecular Gene Lysogeny Antigens Bacterial biology Base Sequence Sequence Homology Amino Acid General Medicine Sequence Analysis DNA biochemical phenomena metabolism and nutrition Lambda phage biology.organism_classification Molecular biology Open reading frame Glucosyltransferases Sequence Alignment Plasmids |
| Zdroj: | Gene. 195(2) |
| ISSN: | 0378-1119 |
| Popis: | With lysogeny by bacteriophage SfV, Shigella flexneri serotype Y is converted to serotype 5a. The glucosyl transferase gene (gtr) from bacteriophage SfV of S. flexneri, involved in serotype-specific conversion, was cloned and characterized. The DNA sequence of a 3.7 kb EcoRI-BamHI fragment of bacteriophage SfV which includes the gtr gene was determined. This gene, encoding a polypeptide of 417 aa with 47.67 kDa molecular mass, caused partial serotype conversion of S. flexneri from serotype Y to type V antigen as demonstrated by Western blotting and the sensitivity of the hybrid strain to phage Sf6. The deduced protein of the partially sequenced open reading frame upstream of the gtr showed similarity to various glycosyl transferases of other bacteria. Orf3, separated from the gtr by a non-coding region and transcribed convergently, codes for a 167 aa (18.8 kDa) protein found to have homology with tail fibre genes of phage lambda and P2. |
| Databáze: | OpenAIRE |
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