Mapping Receptor Antibody Endocytosis and Trafficking in Brain Endothelial Cells
| Autor: | Mikkel R. Holst, Simone S. E. Nielsen, Morten Nielsen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Chemistry Endocytic cycle Drug delivery to the brain Endocytosis 01 natural sciences Antibody labeling Receptor antibody 0104 chemical sciences Cell biology 010404 medicinal & biomolecular chemistry 03 medical and health sciences Drug delivery Image acquisition Receptor 030304 developmental biology |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071616727 Permeability Barrier-Methods and Protocols Holst, M R, Nielsen, S S E & Nielsen, M S 2021, Mapping Receptor Antibody Endocytosis and Trafficking in Brain Endothelial Cells . in Permeability Barrier : Methods and Protocols . 2. edn, Springer, Methods in molecular biology (Clifton, N.J.), vol. 2367, pp. 193-205 . https://doi.org/10.1007/7651_2020_312 Methods in Molecular Biology Methods in Molecular Biology-Permeability Barrier |
| ISSN: | 1064-3745 1940-6029 |
| DOI: | 10.1007/7651_2020_312 |
| Popis: | Drug delivery to the brain is a tremendous problem for the academic society and the industry. One solution with a huge potential is to use endocytic receptors as carriers. Here we describe how endocytic activity and subcellular trafficking of a specific receptor in brain endothelial cells can be characterized in three steps. (1) Labeling, endocytosis, and trafficking of a specific receptor at given time points in a pulse-chase experiment. (2) Fixed antibody labeling and co-staining of subcellular markers for image acquisition. (3) Analysis and quantification of co-localization between the receptor and subcellular markers in ImageJ. |
| Databáze: | OpenAIRE |
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