Development and Characterization of a Nonelectronic Versatile Oxygenating Perfusion System for Tissue Preservation

Autor: Daniel J. Portillo, Jose Gonzalez, Carorina Villarreal, Sophia J. Salazar, Anjelyka Fasci, Brandt Wearden, Jessica Oseghale, Anis Khalil, Thomas Perillo, Leslie Muenchow, Robert De Lorenzo, R. Lyle Hood
Rok vydání: 2022
Předmět:
Zdroj: Annals of Biomedical Engineering. 50:978-990
ISSN: 1573-9686
0090-6964
DOI: 10.1007/s10439-022-02977-2
Popis: Oxygenated machine perfusion of human organs has been shown to improve both preservation quality and time duration when compared to the current gold standard: static cold storage. However, existing machine perfusion devices designed for preservation and transportation of transplantable organs are too complicated and organ-specific to merit use as a solution for all organs. This work presents a novel, portable, and nonelectronic device potentially capable of delivering oxygenated machine perfusion to a variety of organs. An innovative pneumatic circuit system regulates a compressed oxygen source that cyclically inflates and deflates silicone tubes, which function as both the oxygenator and perfusion pump. Different combinations of silicone tubes in single or parallel configurations, with lengths ranging from 1.5 to 15.2 m, were evaluated at varying oxygen pressures from 27.6 to 110.3 kPa. The silicone tubes in parallel configurations produced higher peak perfusion pressures (70% increase), mean flow rates (102% increase), and oxygenation rates (268% increase) than the single silicone tubes that had equivalent total lengths. While pumping against a vascular resistance element that mimicked a kidney, the device achieved perfusion pressures (8.4-131.6 mmHg), flow rates (2.0-40.2 mL min
Databáze: OpenAIRE
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