High expression of PSM-E correlated with tumor grade in prostate cancer: a new alternatively spliced variant of prostate-specific membrane antigen
| Autor: | Xiao-Peng Mao, Lin Xu, Shao-peng Qiu, Kaiyuan Cao, Shu-Qin Dai, Guang-qing Yuan, Dao-hu Wang, Bo-Jian Zheng |
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| Rok vydání: | 2007 |
| Předmět: |
Glutamate Carboxypeptidase II
Male Pathology medicine.medical_specialty Urology Blotting Western Molecular Sequence Data urologic and male genital diseases Immunofluorescence Transfection Statistics Nonparametric Prostate cancer Antigen Prostate Cell Line Tumor LNCaP Glutamate carboxypeptidase II medicine Humans Protein Isoforms RNA Messenger Cloning Molecular medicine.diagnostic_test Base Sequence business.industry Reverse Transcriptase Polymerase Chain Reaction fungi Cancer Prostatic Neoplasms Sequence Analysis DNA medicine.disease Alternative Splicing medicine.anatomical_structure Real-time polymerase chain reaction Oncology Microscopy Fluorescence Antigens Surface Cancer research business Sequence Alignment |
| Zdroj: | The Prostate. 67(16) |
| ISSN: | 0270-4137 |
| Popis: | BACKGROUND Prostate-specific membrane antigen (PSMA) overexpressed in prostate cancer (PCa) has been targeted for therapy and diagnosis of PCa. In the current study, PSMA cDNA was cloned from PCa tissue by RT-PCR. After sequencing, a new spliced variant of PSMA (PSM-E) was discovered and its specificity in PCa was evaluated. METHODS PSM-E and PSMA mRNA were measured in LNCaP, PC-3 and prostate or nonprostatic malignancies. Following transfection of PC-3 with PSM-E cDNA in the pcDNA3.0 vector, PSM-E expression was measured by immunofluorescence and Western-blot. PSM-E and PSMA mRNA levels were quantified by a real-time PCR assay in normal prostate (n = 7), benign prostatic hyperplasia (BPH) (n = 22) and PCa (n = 41). The correlation between their levels and tumor grade was analyzed. RESULTS PSM-E cDNA is identical to PSMA except for a 97-nucleotide region and a 93-nucleotide region. PSM-E and PSMA mRNA were detected in PCa and LNCaP, not in PC-3; PSMA could be detected in some nonprostatic tumors whereas PSM-E not. The expression of PSM-E protein was detected in transfected cells. Significant difference of PSM-E mRNA levels was observed among normal prostate, BPH and PCa (P |
| Databáze: | OpenAIRE |
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