Amplification of AML1 in acute lymphoblastic leukemia is associated with a poor outcome
| Autor: | Louise Harewood, Christine J. Harrison, Kan Luk Cheung, G R Jalali, Zoë J. Broadfield, Susan M. Richards, Chris Mitchell, Anthony V. Moorman, M Martineau, Rachel L. Harris, Kerry E. Taylor, Hazel M. Robinson |
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| Rok vydání: | 2003 |
| Předmět: |
Cancer Research
Time Factors Lymphoblastic Leukemia Biology Disease-Free Survival Immunophenotyping Disease course Proto-Oncogene Proteins Humans Child Gene T-cell receptor Gene Amplification Hematology Gene rearrangement Precursor Cell Lymphoblastic Leukemia-Lymphoma Prognosis Minimal residual disease Neoplasm Proteins DNA-Binding Proteins Treatment Outcome Oncology Child Preschool Core Binding Factor Alpha 2 Subunit Immunology Transcription Factors TAL1 |
| Zdroj: | Leukemia. 17:2249-2250 |
| ISSN: | 1476-5551 0887-6924 |
| Popis: | A total of 28 children and nine adults with relapsed T-ALL were analyzed for the configuration of their T-cell receptor (TCR) and TAL1 genes at diagnosis and relapse to evaluate their stability throughout the disease course. A total of 150 clonal TCR and TAL1 gene rearrangements were identified in the 37 patients at diagnosis. In 65% of cases all rearrangements and in 27% of cases most rearrangements found at diagnosis were preserved at relapse. Two children with unusually late T-ALL recurrences displayed completely different TCR gene rearrangement sequences between diagnosis and relapse. This indicates that a proportion of very late T-ALL recurrences might represent second T-ALL. Specifically, 88% of clonal rearrangements identified at diagnosis in truly relapsed T-ALL were preserved at relapse. This is significantly higher as compared to previously studied precursor-B-ALL (~70%). Thus, from biological point of view, immunogenotype of T-ALL is more stable as compared with precursor-B-ALL. The overall stability of TCR gene rearrangements was higher in adult T-ALL (97%) than in childhood T-ALL (86%). Based on the stability of TCR gene rearrangements, we propose a strategy for PCR target selection (TCRD+TAL1 TCRB TCRG), which probably allows reliable minimal residual disease detection in all T-ALL patients. |
| Databáze: | OpenAIRE |
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