Natural killer T (NKT)–B-cell interactions promote prolonged antibody responses and long-term memory to pneumococcal capsular polysaccharides
| Autor: | Li Bai, Rebecca Mathew, Albert Bendelac, Paul B. Savage, Shenglou Deng, Luc Teyton, Rachel Reboulet |
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| Rok vydání: | 2013 |
| Předmět: |
CD1
chemical and pharmacologic phenomena Cell Communication Antibodies Pneumococcal Infections Statistics Nonparametric Mice Antigen medicine Animals B cell B-Lymphocytes Multidisciplinary CD40 biology Polysaccharides Bacterial Cell Differentiation hemic and immune systems Biological Sciences Flow Cytometry Natural killer T cell Mice Inbred C57BL B-1 cell medicine.anatomical_structure CD1D Immunology biology.protein Nanoparticles Natural Killer T-Cells Antibody Immunologic Memory |
| Zdroj: | Proceedings of the National Academy of Sciences. 110:16097-16102 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1303218110 |
| Popis: | Innate-like natural killer T (NKT) cells critically enhance cell and humoral immunity against infections through recognition of conserved microbial lipid antigens presented by CD1d-expressing antigen-presenting cells, and provision of CD40L and cytokine signals. Whereas NKT cells efficiently licensed dendritic cells to prime potent effector and memory T cells, studies based on model antigens such as alphagalactosylceramide-nitrophenyl conjugates concluded that help to B cells was associated with NKT follicular helper differentiation, but limited to short-term responses without induction of memory. We revisited this surprising conclusion in the context of the extracellular encapsulated pathogen Streptococcus pneumoniae, where recognition of lipid and capsular polysaccharide antigens by NKT cells and B cells, respectively, provide critical host protection. Using liposomal nanoparticles displaying synthetic lipid and polysaccharide antigens to elicit pure and direct NKT-B-cell interactions in vivo, we observed intense and prolonged antibody responses with isotype switch, affinity maturation, and long-lasting B-cell memory, despite modest or absent NKT follicular helper differentiation. Furthermore, conditional ablation of Cd1d demonstrated a requirement for a two-step process involving first cognate interactions with dendritic cells, for NKT cell activation, and then with B cells, for induction of isotype switch and memory. Thus, NKT help to B cells represents both a major arm of antimicrobial defense and a promising target for B-cell vaccines. |
| Databáze: | OpenAIRE |
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