ComEB protein is dispensable for transformation but must be translated for optimal synthesis of ComEC
| Autor: | Jeanette Hahn, David Dubnau, Micaela De Santis |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
DNA
Bacterial Operon Bacillus subtilis Microbiology Article 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Multienzyme Complexes DCMP Deaminase Molecular Biology Post-transcriptional regulation 030304 developmental biology 0303 health sciences biology 030306 microbiology Cell Membrane Membrane Proteins Translation (biology) Cytidine deaminase biology.organism_classification DNA Transformation Competence Ribosomal binding site Cell biology DNA-Binding Proteins Open reading frame Transformation (genetics) chemistry Transformation Bacterial DNA |
| Zdroj: | Mol Microbiol |
| DOI: | 10.1101/2020.12.30.424838 |
| Popis: | We show that the ComEB protein is not required for transformation in Bacillus subtilis, despite its expression from within the comE operon under competence control, nor is it required for the correct polar localization of ComGA. We show further that the synthesis of the putative channel protein ComEC is translationally coupled to the upstream comEB open reading frame, so that the translation of comEB and a suboptimal ribosomal-binding site embedded in its sequence are needed for proper comEC expression. Translational coupling appears to be a common mechanism in three major competence operons for the adjustment of protein amounts independent of transcriptional control, probably ensuring the correct stoichiometries for assembly of the transformation machinery. comEB and comFC, respectively, encode cytidine deaminase and a protein resembling type 1 phosphoribosyl transferases and we speculate that nucleotide scavenging proteins are produced under competence control for efficient reutilization of the products of degradation of the non-transforming strand during DNA uptake. |
| Databáze: | OpenAIRE |
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