High Resolution Imaging of Mouse Embryos and Neonates with X‐Ray Micro‐Computed Tomography
| Autor: | Uchechukwu Akoma, Tara L. Rasmussen, Mary E. Dickinson, Audrey E. Christiansen, Sowmya Kalaga, Chih-Wei Hsu |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Materials science Micro computed tomography Iodine Compounds X-ray Contrast Media Soft tissue Embryo X-Ray Microtomography General Medicine Embryo Mammalian Article Mice 03 medical and health sciences 0302 clinical medicine Animals Newborn Simple sample Animals Tomography Postnatal day High resolution imaging 030217 neurology & neurosurgery 030304 developmental biology Biomedical engineering |
| Zdroj: | Curr Protoc Mouse Biol |
| ISSN: | 2161-2617 |
| Popis: | Iodine-contrast micro-computed tomography (microCT) 3D imaging provides a non-destructive and high-throughput platform for studying mouse embryo and neonate development. Here we provide protocols on preparing mouse embryos and neonates between embryonic day 8.5 (E8.5) to postnatal day 4 (P4) for iodine-contrast microCT imaging. With the implementation of the STABILITY method to create a polymer-tissue hybrid structure, we have demonstrated that not only is soft tissue shrinkage minimized but also the minimum required time for soft tissue staining with iodine is decreased, especially for E18.5 to P4 samples. In addition, we also provide a protocol on using commercially available X-CLARITYTM hydrogel solution to create the similar polymer-tissue hybrid structure on delicate early post-implantation stage (E8.5 to E14.5) embryos. With its simple sample staining and mounting processes, this protocol is easy to adopt and implement for most of the commercially available, stand-alone microCT systems in order to study mouse development between early post-implantation to early postnatal stages. © 2019 by John Wiley & Sons, Inc. |
| Databáze: | OpenAIRE |
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