Biochemical characterization of extracellular polymeric substances from endodontic biofilms

Autor: Grettel Valle-Bourrouet, Cristina Retana-Lobo, Tatiana Ramirez-Mora
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Exopolysaccharides
Microorganism
Cell Membranes
Glycobiology
lcsh:Medicine
Pathology and Laboratory Medicine
Biochemistry
Physical Chemistry
Fatty Acids
Monounsaturated
0302 clinical medicine
Medicine and Health Sciences
lcsh:Science
chemistry.chemical_classification
Multidisciplinary
biology
Chemistry
Fatty Acids
Biofilm matrix
Lipids
Extracellular Matrix
Bacterial Pathogens
Medical Microbiology
Physical Sciences
Carbohydrate Metabolism
Cellular Structures and Organelles
Pathogens
Oxidoreductases
Research Article
Surface Chemistry
030106 microbiology
Carbohydrates
Enterococcus Faecalis
Microbiology
Enterococcus faecalis
03 medical and health sciences
Extracellular polymeric substance
Bacterial Proteins
Polysaccharides
Actinomyces
Humans
Microbial Pathogens
Bacteria
lcsh:R
Biofilm
Organisms
Fatty acid
Biology and Life Sciences
Membrane Proteins
Bacteriology
030206 dentistry
Cell Biology
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Outer Membrane Proteins
Biofilms
Actinomyces naeslundii
Artificial Membranes
lcsh:Q
Bacterial Biofilms
Enterococcus
Periapical Periodontitis
Molecular Chaperones
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 11, p e0204081 (2018)
ISSN: 1932-6203
Popis: Apical periodontitis is frequently associated with the presence of bacteria biofilm, which has an indisputable impact on the prognosis of endodontic therapy due to the high resistance to adverse environmental conditions, chemicals, and antibiotic therapy that characterize bacteria within biofilm. The biofilm matrix acts as a protective shield over the encased microorganisms. The aim of this investigation was to identify the main biochemical components of biofilm matrix from endodontic mono- and dual-species biofilms. Enterococcus faecalis and Actinomyces naeslundii were cultured as mono- and dual-species biofilms for 14 days. Crude extracellular polymeric substances (EPSs) from biofilm matrices were extracted using chemical and physical methods. High-performance liquid chromatography, gas chromatography, and mass spectrometry were used to determine the carbohydrate, protein, and fatty acid components. Chemical analysis of the biofilm matrices revealed that they were mainly composed of stachyose, maltose, and mannose carbohydrates. The protein profile in all biofilm samples showed abundant oxidoreductases and chaperone proteins and some virulence- associated proteins mainly located in the membrane surface. High percentages of saturated and monounsaturated fatty acids were identified in all biofilm matrices, with a major prevalence of palmitic, stearic, and oleic acids. Based on the results, it was possible to obtain for the first time a general overview of the biochemical profile of endodontic biofilm matrices.
Databáze: OpenAIRE
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