Arabidopsis mutants with increased organ regeneration in tissue culture are more competent to respond to hormonal signals
| Autor: | Rafaël Smets, Shyam J. Uttamchandani, Andrew J. Cary, Stephen H. Howell, Harry Van Onckelen |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Cytokinins
Arabidopsis Organogenesis Plant Science Biology Plant Roots chemistry.chemical_compound Tissue culture Plant Growth Regulators Chromosome Segregation Culture Techniques Botany Genetics Indoleacetic Acids Arabidopsis Proteins fungi food and beverages Meristem biology.organism_classification Cell biology Complementation Micropropagation chemistry Cytokinin Mutation Protein Kinases Cell Division Plant Shoots Explant culture Signal Transduction |
| Zdroj: | Planta |
| ISSN: | 0032-0935 |
| Popis: | Shoots and roots can be regenerated through organogenesis in tissue culture by subjecting plant explants to the appropriate regime of hormone treatments. In an effort to understand the control of shoot organogenesis, we screened for mutants in Arabidopsis thaliana (L.) Heynh. Columbia ecotype for enhanced shoot development at sub-optimal concentrations of cytokinin. Mutants in four different complementation groups were identified, one of which represents a new locus named increased organ regeneration1 (ire1) and another that is allelic to the previously identified pom1/erh2 mutant. Although the mutants were selected for their response to cytokinin, they were neither hypersensitive to, nor were they over-producers of cytokinins. The mutations identified in this study not only promote more robust shoot production in tissue culture, but also enhance green-callus and root formation. We interpret this to mean that, in tissue culture, IRE genes act before organ specification during the time when root explants acquire the competency to respond to organ formation signals. In normal plant development, IRE genes may down-regulate the competency of vegetative tissue to respond to hormonal signals involved in shoot and root organogenesis. |
| Databáze: | OpenAIRE |
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