Induction of apoptosis/necrosis in various human cell lineages by Haemophilus ducreyi cytolethal distending toxin
| Autor: | Teresa Lagergård, Jozef Azem, Catharina Wising, Karin Ahlman, Madeleine Zetterberg, Liselott A. Svensson |
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| Rok vydání: | 2005 |
| Předmět: |
Keratinocytes
Programmed cell death Necrosis Cytolethal distending toxin T cell Bacterial Toxins Apoptosis Phosphatidylserines Biology Toxicology Jurkat cells Statistics Nonparametric Microbiology Haemophilus ducreyi chemistry.chemical_compound Annexin Cell Line Tumor medicine Humans Myeloid Cells Propidium iodide Annexin A5 Dose-Response Relationship Drug Caspase 3 Epithelial Cells Fibroblasts Flow Cytometry Molecular biology Protein Transport medicine.anatomical_structure chemistry Caspases medicine.symptom Propidium |
| Zdroj: | Toxicon. 45:767-776 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/j.toxicon.2005.01.016 |
| Popis: | We investigated the impact of highly purified Haemophilus ducreyi cytolethal distending toxin (HdCDT) on the apoptosis and necrosis of various human cells; including myeloid cells, epithelial cells, keratinocytes, and primary fibroblasts. The levels of apoptosis and necrosis induced in these cells were compared to those induced by HdCDT in human T cells and in the Jurkat T cell line. Levels of caspase-3 activity were measured, and membrane changes like phosphatidylserine (PS) translocation was evaluated after double-staining with the fluorescein isothiocyanate (FITC)-labeled annexin V and propidium iodide (PI) using flow cytometry. HdCDT induced various degrees of apoptosis and necrosis in dose- and time-dependent manners in cells of various lineages. Early and late apoptosis (annexin V-stained cells) were induced in more than 90% of T cells and monocytes after treatment with 100 ng/ml HdCDT for 24 and 48 h, respectively. The corresponding numbers for epithelial cells, keratinocytes, and fibroblasts were 26-32% after treatment with 100 ng/ml HdCDT for 48 h. HdCDT appears to eliminate effectively by inducing apoptosis those cells that are involved in immune responses. Epithelial cells, keratinocytes and fibroblasts, which are important for the healing of chancroid ulcers, are eliminated by apoptosis or necrosis after contact with HdCDT, albeit slower and to a lesser extent than T cells. |
| Databáze: | OpenAIRE |
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