Rapid Determination of L-carnitine in Infant and Toddler Formulas by Liquid Chromatography Tandem Mass Spectrometry
| Autor: | Jung-Min Park, Na-Kyeoung Kim, Jang-Hyuk Ahn, Jin Man Kim, Byung-Man Kwak |
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| Rok vydání: | 2014 |
| Předmět: |
Chromatography
Chloroform Chemistry Electrospray ionization Analytical chemistry analytical method infant formula Hydrochloric acid Article Ion chemistry.chemical_compound Certified reference materials Infant formula Liquid chromatography–mass spectrometry toddler formula L-carnitine Animal Science and Zoology LC-MS/MS Toddler Food Science |
| Zdroj: | Korean Journal for Food Science of Animal Resources |
| ISSN: | 1225-8563 |
| DOI: | 10.5851/kosfa.2014.34.6.749 |
| Popis: | A rapid and simple analytical method for L-carnitine was developed for infant and toddler formulas by liquid chromatography tandem mass spectrometry (LC-MS/MS). A 0.3 g of infant formula and toddler formula sample was mixed in a 50 mL conical tube with 9 mL water and 1 mL 0.1 M hydrochloric acid (HCl) to chemical extraction. Then, chloroform was used for removing a lipid fraction. After centrifuged, L-carnitine was separated and quantified using LC-MS/MS with electrospray ionization (ESI) mode. The precursor ion for L-carnitine was m/z 162, and product ions were m/z 103 (quantitative) and m/z 85 (qualitative), respectively. The results for spiked recovery test were in the range of 93.18-95.64% and the result for certified reference material (SRM 1849a) was within the range of the certificated values. This method could be implemented in many laboratories that require time and labor saving. |
| Databáze: | OpenAIRE |
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