Immunohistochemistry of articular cartilage from immature beagle dogs dosed with difloxacin
| Autor: | John E. Burkhardt, Ralf Stahlmann, E. Lozo, Michael A. Hill, Christian Förster |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Cartilage
Articular Pathology medicine.medical_specialty 040301 veterinary sciences Administration Oral Toxicology 030226 pharmacology & pharmacy Beagle Chondrocyte Pathology and Forensic Medicine 0403 veterinary science 03 medical and health sciences 0302 clinical medicine Dogs Anti-Infective Agents Antigens CD Ciprofloxacin medicine Animals Molecular Biology Difloxacin Antibacterial agent biology Chemistry Cartilage 04 agricultural and veterinary sciences Cell Biology Anatomy Humerus Integrin alphaV Arthritis Experimental Immunohistochemistry Staining Extracellular Matrix Fibronectins Fibronectin medicine.anatomical_structure biology.protein Collagen medicine.drug Fluoroquinolones |
| Zdroj: | Toxicologic pathology. 25(5) |
| ISSN: | 0192-6233 |
| Popis: | Effects of the fluoroquinolone difloxacin on articular-epiphyseal cartilage in growing beagle dogs have been described by light microscopic, electron microscopic, and biochemical methods. Here we present data from an immunohistochemistry study with humeral head cartilage from 3-mo-old beagle dogs after treatment with 1 or 2 oral doses of 300 mg difloxacin/kg body weight. Dogs were euthanatized either 24 hr (single dose) or 48 hr (2 doses) after onset of dosing, and cartilage tissue was stored at -90°C until it was studied by immunohistochemistry. Antibodies against matrix components (collagen II, fibronectin) as well as antibodies against cellular structures (integrins) were used. After single-dose treatment (24-hr group), cartilage lesions such as clefts were not observed, but increased staining for fibronectin was found in cartilage samples from 5 of 6 animals. Markedly increased staining for fibronectin was also demonstrated in the vicinity of clefts within cartilage of all animals of the 48-hr group. Collagen II staining was homogeneously distributed in cartilage from controls and was slightly reduced in territorial matrix in 2 of 6 dogs of the 48-hr group. Integrin staining on chondrocytes was not significantly affected by difloxacin under the given conditions with the exception of a slight reduction of the α v integrin chain in 1 of 5 dogs of the 48-hr group. Overall, the most important result is the finding that fibronectin was a sensitive immunohistochemical marker for change in cartilage samples due to difloxacin treatment in dogs. |
| Databáze: | OpenAIRE |
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