PTENallelic loss is an important mechanism in the late stage of development of oral leucoplakia into oral squamous cell carcinoma
| Autor: | Ligia Akiko Ninokata Miyahara, Felipe Paiva Fonseca, Hélder Antônio Rebelo Pontes, Flávia Sirotheau Corrêa Pontes, Rommel Rodríguez Burbano, Nicolau Conte Neto, Douglas Magno Guimarães |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Male Mild Dysplasia Pathology medicine.medical_specialty Histology Cell Biology Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine medicine Humans PTEN Alleles Aged Leukoplakia Aged 80 and over Squamous Cell Carcinoma of Head and Neck PTEN Phosphohydrolase Late stage Cancer 030206 dentistry General Medicine Middle Aged medicine.disease stomatognathic diseases Cell Transformation Neoplastic medicine.anatomical_structure Head and Neck Neoplasms Dysplasia 030220 oncology & carcinogenesis Carcinoma Squamous Cell Cancer research biology.protein Immunohistochemistry Female Mouth Neoplasms Leukoplakia Oral Precancerous Conditions |
| Zdroj: | Histopathology. 72:330-338 |
| ISSN: | 0309-0167 |
| DOI: | 10.1111/his.13381 |
| Popis: | Objectives The aim of this study is to analyse allelic loss of the PTEN gene and its protein immunoexpression in dysplastic oral lesions and oral squamous cell carcinomas (OSCCs). Materials And Methods Samples were collected from 153 patients (20 ranulas used as a control – C, 30 leukoplakias with mild dysplasia – MD, 30 leukoplakias with moderate to severe dysplasia – MSD, and 73 OSCC). PTEN protein expression was investigated using immunohistochemistry, and PTEN allelic loss was analysed by fluorescence in situ hybridisation (FISH). Differences among groups were evaluated using the Chi-square test. Results PTEN expression was higher in MSD (p=0.002) and OSCC (p=0.0259) compared with the C group; additionally, a higher expression was observed in MSD (p=0.0035) and OSCC (p=0.049) than MD. Regarding FISH analysis, a higher hemizygous (single copy) loss was observed in OSCC than C (p=0.0467) and OSCC than MD (p=0.0175), as well as a higher homozygous deletion in OSCC compared with C (p=0.0159) and OSCC than MD (p=0.0145). Conclusion The results of this work suggest that PTEN allelic loss is an important mechanism in the late stage of the development of oral potentially malignant lesions into oral cancer. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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