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Fungal endophytes are microfungi that live in plants without causing apparent symptoms of infection. The purpose of this work was to identify the endophytic fungi isolated from the medicinal plant Carpobrotus edulis collected in the Atlantic coast region of Rabat city in Morocco. The isolates were obtained from different parts of the plant (flowers, stems and leaves). A total 12 isolates were characterized and identified using molecular biology analysis based on the internal transcribed spacer (ITS) region sequencing. 5 fungal species of endophytic fungi were successfully isolated from Carpobrotus edulis including Trichoderma asperellum, Fusarium culmorum, Penicillium radicum, Aspergillus flavus and 8 isolates represented the same fungal species Aspergillus fumigatus. The 5 fungal species isolated in the current study are reported for the first time as Carpobrotus edulis endophytes. Key words: Fungal endophytes, Carpobrotus edulis, ITS region Sequencing REFERENCES Arnold, A. 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