Specific Recognition of Human Influenza Virus with PEDOT Bearing Sialic Acid-Terminated Trisaccharides
| Autor: | Wenfeng Hai, Akira Matsumoto, Yuji Miyahara, Hiroaki Takeuchi, Yukichi Horiguchi, Shoji Yamaoka, Tatsuro Goda |
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| Rok vydání: | 2017 |
| Předmět: |
Conductive polymer
Materials science biology Hemagglutinin (influenza) 02 engineering and technology Quartz crystal microbalance 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Combinatorial chemistry 0104 chemical sciences Contact angle Molecular recognition biology.protein Moiety Organic chemistry General Materials Science Cyclic voltammetry 0210 nano-technology Biosensor |
| Zdroj: | ACS Applied Materials & Interfaces. 9:14162-14170 |
| ISSN: | 1944-8252 1944-8244 |
| Popis: | Conducting polymers are good candidates for biosensor applications when molecular recognition element is imparted. We developed trisaccharide-grafted conducting polymers for label-free detection of the human influenza A virus (H1N1) with high sensitivity and specificity. A 3,4-ethylenedioxythiophene (EDOT) derivative bearing an oxylamine moiety was electrochemically copolymerized with EDOT. The obtained film was characterized by cyclic voltammetry, X-ray photoelectron spectroscopy, scanning electron microscopy, stylus surface profilometer, and AC-impedance spectroscopy. The trisaccharides comprising Sia-α2,6'-Gal-Glu (2,6-sialyllactose) or Sia-α2,3'-Gal-Glu (2,3-sialyllactose) were covalently introduced to the side chain of the conducting polymers as a ligand for viral recognition. Immobilization of sialyllactose was confirmed by quartz crystal microbalance (QCM) and water contact angle measurements. Specific interaction of 2,6-sialyllactose with hemagglutinin in the envelope of the human influenza A virus (H1N1) was detected by QCM and potentiometry with enhanced sensitivity by 2 orders of magnitude when compared with that of commercially available kits. The developed conducting polymers possessing specific virus recognition are a good candidate material for wearable monitoring and point-of-care testing because of their processability and mass productivity in combination with printing technologies. |
| Databáze: | OpenAIRE |
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