Conventional Light Chains Inhibit the Autonomous Signaling Capacity of the B Cell Receptor
| Autor: | Thomas Wossning, Fabian Kohler, Markus Müschen, Sonja Meixlsperger, Michael Reppel, Hassan Jumaa |
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| Rok vydání: | 2007 |
| Předmět: |
Immunoglobulin Light Chains
Surrogate B-cell receptor Immunology Receptors Antigen B-Cell Biology Ligands Immunoglobulin light chain Mice Cell Line Tumor medicine Animals Humans Immunology and Allergy Phosphorylation MOLIMMUNO Receptor B cell Adaptor Proteins Signal Transducing B-Lymphocytes Membrane Glycoproteins Signal transducing adaptor protein Molecular biology Cell biology medicine.anatomical_structure Infectious Diseases Cell culture Calcium Immunoglobulin Light Chains Signal transduction Signal Transduction |
| Zdroj: | Immunity. 26(3):323-333 |
| ISSN: | 1074-7613 |
| DOI: | 10.1016/j.immuni.2007.01.012 |
| Popis: | Signals from the B cell antigen receptor (BCR), consisting of mu heavy chain (muHC) and conventional light chain (LC), and its precursor the pre-BCR, consisting of muHC and surrogate light chain (SLC), via the adaptor protein SLP-65 regulate the development and function of B cells. Here, we compare the effect of SLC and conventional LC expression on receptor-induced Ca(2+) flux in B cells expressing an inducible form of SLP-65. We found that SLC expression strongly enhanced an autonomous ability of muHC to induce Ca(2+) flux irrespective of additional receptor crosslinking. In contrast, LC expression reduced this autonomous muHC ability and resulted in antigen-dependent Ca(2+) flux. These data indicate that autonomous ligand-independent signaling can be induced by receptor forms other than the pre-BCR. In addition, our data suggest that conventional LCs play an important role in the inhibition of autonomous receptor signaling, thereby allowing further B cell differentiation. |
| Databáze: | OpenAIRE |
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